Objective  To investigate tegument antigens and intestine-associated antigens of Pagumogonimus skrjabini (P. skrjabini) adult worm and provide the immunodiagnostic antigen for clinical diagnosis.   Methods  The adults of P. skrjabini were sectioned at a thickness of 15 μm. Serum specific antigens from patients with P. skrjabini infection was used for immunological study. The immunodiagnosis-associated antigens were identified by using immunogold techniques

immune electron microscopy

enzyme-labeled immunoassay and laser scanning confocal microscopy.   Results  Both immunogold techniques and enzyme-labeled immunoassay showed that specific antigens were concentrated in the tegument and enteric cavity. Confocal laser scanning microscopy showed that semi-quantitative analysis of tegument antigen of average gray value was 80.65

and that of intestine antigen was 71.25. At the ultrastructural level

the immunogold labeling showed deposit of gold particles on the surface membrane of tegument and enteric cavity.   Conclusion  Identification of adult specifical antigens of P. skrjabini by using paragonimiasis sera are tegument- and intestine-associated antigens

therefore

immunodiagnostics of paragonimiasis are the adult components of the surface membrane and intestine.