Cotransplantation of human umbilical cord blood-derived stromal cells and human umbilical cord blood-mononuclear cells accelerates hematopoietic reconstruction and engraftment in radiation-injured mice

刘颖; 陈幸华; 张曦; 高蕾; 张诚; 郝磊; 冯一梅

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Cotransplantation of human umbilical cord blood-derived stromal cells and human umbilical cord blood-mononuclear cells accelerates hematopoietic reconstruction and engraftment in radiation-injured mice

更新时间：2026-01-09

- Cotransplantation of human umbilical cord blood-derived stromal cells and human umbilical cord blood-mononuclear cells accelerates hematopoietic reconstruction and engraftment in radiation-injured mice
- Journal of Army Medical University Vol. 32, Issue 9, Pages: 895-899(2010)
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  CLC： R329.2;R457.7;R55
- Published：2010
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刘颖,陈幸华,张曦,高蕾,张诚,郝磊,冯一梅.人脐血源基质细胞联合造血细胞共移植促进造血重建与植入的研究[J].陆军军医大学学报(原第三军医大学学报),2010,32(09):895-899.

　Liu Ying,Chen Xinghua,Zhang Xi,Gao Lei,Zhang Cheng,Hao Lei,Feng Yimei.Cotransplantation of human umbilical cord blood-derived stromal cells and human umbilical cord blood-mononuclear cells accelerates hematopoietic reconstruction and engraftment in radiation-injured mice[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(09):895-899.
刘颖,陈幸华,张曦,高蕾,张诚,郝磊,冯一梅.人脐血源基质细胞联合造血细胞共移植促进造血重建与植入的研究[J].陆军军医大学学报(原第三军医大学学报),2010,32(09):895-899. DOI：

　Liu Ying,Chen Xinghua,Zhang Xi,Gao Lei,Zhang Cheng,Hao Lei,Feng Yimei.Cotransplantation of human umbilical cord blood-derived stromal cells and human umbilical cord blood-mononuclear cells accelerates hematopoietic reconstruction and engraftment in radiation-injured mice[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(09):895-899. DOI：

摘要

目的探讨人脐血源基质细胞(human umbilical cord blood-derived stromal cells

hUCBDSCs)联合脐血单个核细胞(human umbilical cord blood-mononuclear cells

hUCB-MNCs)共移植促进放射损伤BALB/c小鼠造血重建与植入的效应。方法 BALB/c近交系小鼠经

Coγ射线8.5 Gy辐照后1 d按照完全随机设计分为4组，经尾静脉分别输注生理盐水、hUCB-MNCs（1×10

/只）、hUCB-MNCs（1×10

/只）联合hUCBDSCs（1×10

/只）及hUCB-MNCs（1×10

/只）联合人骨髓基质细胞（hBMSCs，1×10

/只）。检测移植后各组小鼠存活率、外周血象、骨髓病理变化、成纤维细胞集落形成单位(CFU-F)和脾集落形成单位（CFU-S）计数及人源CD45

细胞植入率、归巢示踪等指标。结果采用hUCBDSCs或hBMSCs联合移植造血受抑轻，恢复快，存活率较单移植组(45%)显著提高(P0.05)，分别为75%和70%。尤其以hUCBDSCs共移植组促血小板恢复作用显著加强，并于移植后第21天恢复至照射前水平。hUCBDSCs共移植组在移植后14 d CFU-F（70.40±4.34/孔）、移植后21 d CFU-F（94.20±8.44/孔）和移植后12 d CFU-S计数（34.75±4.50/只）、CD45

细胞植入率（34.19±2.60）%也显著高于其余组（P0.05）。CM-DiI标记的hUCB-MNCs在体内主要归巢至骨髓和脾脏，并以hUCBDSCs共移植组小鼠骨髓中的含量（85.20±7.89）最多（P0.05）。结论 hUCBDSCs联合hUCB-MNCs共移植能加速小鼠造血重建，提高植入率。

Abstract

Objective To study the effect of co-transplantation of human umbilical cord blood-d

erived stromal cells (hUCBDSC) and human umbilical cord bloodmononuclear cells (hUCB-MNC) on hematopoietic reconstruction and engraftment in BALB/c mice with radiation damage. Methods One day after irradiation with

Coγ at 8.5 Gy

the animals were randomly divided into 4 groups

which were infused with saline

hUCB-MNC（1×10

per mouse）

hUCB-MNC（1×10

per mouse）and hUCBDSC（1×10

per mouse）

and hUCB-MNC (1×10

per mouse）and hBMSC （1×10

per mouse）respectively

through their tail vein. Survival rate

peripheral hemogram

marrow pathological change

CFU-F

CFU-S

engraftment rate

and homing tracing in each group were detected

respectively. Results The survival rate of mice was 75% and 70%，respectively

after cotransplantation of hUCBMNC and hUCBDSC or hBMSC（P0.05）

which was significantly higher than the only hUCB-MNCs group. The number of platelets was increased in the hUCBDSC plus hUCB-MNC group and became normal 21 d after operation. CFU-F

（70.40±4.34 /well）

CFU-F

（94.20±8.44 /well）

CFU-S

（34.75±4.50 per mouse） and the engraftment rate (34.19±2.60)% were significantly higher in the hUCBDSC co-transplantation group than in other groups (P0.05). hUCB-MNC labeled by CM-DiI were mainly distributed in bone marrow and spleen

especially in hUCBDSC plus hUCB-MNC group（85.20±7.89）(P0.05). Conclusion Co-transplantation of hUCBDSC and hUCB-MNCs can significantly promote hematopoietic reconstruction and elevate the engraftment rate.

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