Injection of extracellular domain of p75<sup>NTR</sup> improves proliferation of hippocampal neural stem cells and learning and memory of Alzheimer’s disease mice

高长越; 方传勤; 许志强; 李静; 邓娟; 王延江; 周华东

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Injection of extracellular domain of p75^NTR improves proliferation of hippocampal neural stem cells and learning and memory of Alzheimer’s disease mice

更新时间：2026-01-09

- Injection of extracellular domain of p75^NTR improves proliferation of hippocampal neural stem cells and learning and memory of Alzheimer’s disease mice
- Journal of Army Medical University Vol. 32, Issue 11, Pages: 1152-1154(2010)
- 作者机构：
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  CLC： R749.160.2;R322.81;R329.28
- Published：2010
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高长越,方传勤,许志强,李静,邓娟,王延江,周华东.阻断内源性P75NTR对阿尔茨海默病小鼠海马神经干细胞增殖与学习记忆的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(11):1152-1154.

　Gao Changyue,Fang Chuanqin,Xu Zhiqiang,Li Jing,Deng Juan,Wang Yanjiang,Zhou Huadong.Injection of extracellular domain of p75NTR improves proliferation of hippocampal neural stem cells and learning and memory of Alzheimer’s disease mice[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(11):1152-1154.
高长越,方传勤,许志强,李静,邓娟,王延江,周华东.阻断内源性P75NTR对阿尔茨海默病小鼠海马神经干细胞增殖与学习记忆的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(11):1152-1154. DOI：

　Gao Changyue,Fang Chuanqin,Xu Zhiqiang,Li Jing,Deng Juan,Wang Yanjiang,Zhou Huadong.Injection of extracellular domain of p75NTR improves proliferation of hippocampal neural stem cells and learning and memory of Alzheimer’s disease mice[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(11):1152-1154. DOI：

摘要

目的研究阿尔茨海默病(Alzheimer’s disease

AD)小鼠侧脑室注射p75

NTR

胞外段(extracellular domain of p75

NTR

p75

NTR-ECD

) 阻断内源性p75

NTR

激活后对海马神经干细胞增殖和学习记忆影响。方法选取12月龄APP/PS1双转基因AD小鼠16只，注射组（n=8）连续7 d 侧脑室注射p75

NTR-ECD

（1 μg/d），对照组（n=8）向侧脑室注射等量生理盐水，Morris 水迷宫检测空间记忆，腹腔注射BrdU检测海马齿回神经干细胞增值变化。结果与对照组比较，注射组小鼠海马齿回BrdU阳性细胞数显著增加[（286.39±26.17）vs（142.01±18.12），P0.01

]

，行为学检测显示逃逸潜伏期明显缩短[（30.39±4.31） s vs （39.58±6.19） s

P0.05

]

、穿过原平台位置次数和在原平台象限探索时间百分率显著增加 [（3.72±0.45）vs（2.54±0.32）；（39.58±6.19）% vs （30.42±4.31）% ；P0.05

]

。结论阻断内源性p75

NTR

激活可促进AD小鼠海马神经发生并改善AD小鼠学习记忆。

Abstract

Objective To observe the effect of injecting extracellular domain of p75

NTR

(p75

NTR-ECD

) on learning and memory of APP/PS1 transgenic Alzheimer’s disease (AD) mice and the proliferation of neural stem cells (NSCs) in the hippocampus. Methods Totally 16 12-months-old APP/PS1 transgenic Alzheimer’s disease (AD) mice were divided randomly into expe

rimental group and control group. In the experimental group

p75

NTR-ECD

was injected into lateral cerebral ventricle of AD mice for 7 d (1 μg/d)

and saline was injected in the control group. BrdU immunohistochemistry was used to observe NSCs proliferation in the hippocampus and Morris water maze test was performed to detect the praxiology of AD mice. Results NSCs proliferation in the hippocampus was obviously increased compared with control group [(286.39±26.17) vs (142.01±18.12)

P0.01

]

. In Morris water maze test

escape latency was markedly decreased [(30.39±4.31) vs (39.58±6.19)s

P0.05

]

and the times of crossing the situation of platform and the time percent of swimming in northeast obviously increased in the experimental group [(3.72±0.45) vs (2.54±0.32)

(39.58±6.19)% vs (30.42±4.31)%

P0.05

]

. Conclusion Blocking of p75

NTR

activation by p75

NTR-ECD

enhances hippocampal neurogenesis and improves the abilities of learning and memorization of AD mice.

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Injection of extracellular domain of p75NTR improves proliferation of hippocampal neural stem cells and learning and memory of Alzheimer’s disease mice

DOI：

摘要

Abstract

关键词

Keywords

references

Injection of extracellular domain of p75^NTR improves proliferation of hippocampal neural stem cells and learning and memory of Alzheimer’s disease mice