Effect of Astragalus Membranaceus and Angelica Sinensis on plasminogen activator inhibitor-1 in cultured endothelial cells

杨长春; 马增春

您当前的位置：

首页 >

文章列表页 >

Effect of Astragalus Membranaceus and Angelica Sinensis on plasminogen activator inhibitor-1 in cultured endothelial cells

更新时间：2026-01-09

- Effect of Astragalus Membranaceus and Angelica Sinensis on plasminogen activator inhibitor-1 in cultured endothelial cells
- Journal of Army Medical University Vol. 32, Issue 11, Pages: 1149-1151(2010)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R282.71;R364.15;R392.2
- Published：2010
- 稿件说明：
移动端阅览
杨长春,马增春.黄芪、当归对血管内皮细胞纤溶酶原激活物抑制剂-1的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(11):1149-1151.

　Yang Changchun,Ma Zengchun.Effect of Astragalus Membranaceus and Angelica Sinensis on plasminogen activator inhibitor-1 in cultured endothelial cells[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(11):1149-1151.
杨长春,马增春.黄芪、当归对血管内皮细胞纤溶酶原激活物抑制剂-1的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(11):1149-1151. DOI：

　Yang Changchun,Ma Zengchun.Effect of Astragalus Membranaceus and Angelica Sinensis on plasminogen activator inhibitor-1 in cultured endothelial cells[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(11):1149-1151. DOI：

摘要

目的观察中药黄芪、当归对血管内皮细胞纤溶酶原激活物抑制剂-1（plasminogen activator inhibitor-1，PAI-1）表达及活性的影响，探讨黄芪、当归抗血栓作用的分子机制。方法使用成品的黄芪和当归注射液，各设两个浓度（6 mg/ml和 3 mg/ml），分别加入细胞培养板，实验组各孔均加入0.5 ng/ml TGF-β1作为诱导剂以增加PAI-1Ag表达。同时设立TGF-β1组（细胞液中只加入0.5 ng/ml TGF-β1）以及空白对照组（细胞液中不加入任何成分），各组在37 ℃，5%CO

孵箱中培养24 h。应用RT-PCR方法检测黄芪、当归对血管内皮细胞PAI-1 mRNA表达的影响，ELISA法检测黄芪、当归对PAI-1表达的影响，发色底物显色法检测黄芪、当归对PAI-1活性的影响。结果黄芪、当归可不同程度地降低血管内皮细胞PAI-1 mRNA表达、抗原水平与活性，以黄芪、当归联合应用作用更为明显。黄芪、当归单独及联合应用对PAI-1 mRNA表达的抑制率分别为17%、24.6%和36.6%。结论黄芪、当归可能通过抑制血管内皮细胞PAI-1表达和活性而发挥其抗血栓形成的作用。

Abstract

Objective To investigate the effect of Astragalus Membranaceus(Ast) and Angelica Sinensis(Ang) on antithrombosis and explore the molecular mechanism. Methods Plasminogen activator inhibitor-1(PAI-1) expression and activity were selected to observe the antithrombosis effect of Ast and Ang (3 and 6 mg/ml) on 0.5 ng/ml TGF-β

treated endothelial cells. PAI-1mRNA expression was detected by RT-PCR

PAI-1 antigen assay was detected by specific enzyme-linked immunosorbent assays(ELISA)

and PAI-1 activity was measured by amidolytical assay. Results Ast and Ang significantly inhibited PAI-1 mRNA expreesion

antigen and activity in endothelial cells. The more powerful effects could be observed when treated by Ast and Ang together. Conclusion The PAI-1 mRNA expression，antigen and activity is inhibited effectively by Ast and Ang

which may be the mechanism of their treatment for antithrombosis.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effect of LPS on vascular permeability of vascular endothelial cells and role of connexin43 in the process

切应力诱导大鼠颈动脉内皮细胞组织因子表达的检测与分析

能量可控陡脉冲对人脐静脉血管内皮细胞的体外作用

严重烫伤早期兔血脑屏障病理学观察

内毒素上调血管内皮细胞11β-HSD2基因的表达

Related Author

明佳

袁侨英

周艳

苟元彬

刘良明

杨益民

应大君

李黔宁

Related Institution

No data

⁰