Endothelial progenitor cells combined implantation improves proliferation and suppresses apoptosis of neural  stem cells and angiogenesis of MCAO/R rats

富奇志; 齐志国; 朱晓峰; 谢鹏

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Endothelial progenitor cells combined implantation improves proliferation and suppresses apoptosis of neural stem cells and angiogenesis of MCAO/R rats

更新时间：2026-01-09

- Endothelial progenitor cells combined implantation improves proliferation and suppresses apoptosis of neural stem cells and angiogenesis of MCAO/R rats
- Journal of Army Medical University Vol. 32, Issue 6, Pages: 511-515(2010)
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  CLC： R322.81;R329.28;R743.31
- Published：2010
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富奇志,齐志国,朱晓峰,谢鹏.血管内皮祖细胞对共同植入缺血再灌注模型大鼠脑内的神经干细胞增殖、凋亡和血管形成的调节[J].陆军军医大学学报(原第三军医大学学报),2010,32(06):511-515.

　Fu Qizhi,Qi Zhiguo,Zhu Xiaofeng,Xie Peng.Endothelial progenitor cells combined implantation improves proliferation and suppresses apoptosis of neural stem cells and angiogenesis of MCAO/R rats[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(06):511-515.
富奇志,齐志国,朱晓峰,谢鹏.血管内皮祖细胞对共同植入缺血再灌注模型大鼠脑内的神经干细胞增殖、凋亡和血管形成的调节[J].陆军军医大学学报(原第三军医大学学报),2010,32(06):511-515. DOI：

　Fu Qizhi,Qi Zhiguo,Zhu Xiaofeng,Xie Peng.Endothelial progenitor cells combined implantation improves proliferation and suppresses apoptosis of neural stem cells and angiogenesis of MCAO/R rats[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(06):511-515. DOI：

摘要

目的研究血管内皮祖细胞（endothelial progenitor cells

EPCs）与神经干细胞（neuron stem cells

NSCs）构建的复合移植体对移植入缺血再灌注模型大鼠缺血半暗带的神经干细胞增殖、凋亡和血管重建的影响。方法线栓法建大鼠脑缺血再灌注模型；血管内皮祖细胞、层粘连蛋白和神经干细胞构建的移植复合体移入模型鼠脑缺血半暗带；免疫组化观察Brdu标记的移入脑内神经干细胞和缺血半暗带血管新生，免疫荧光双标观察神经干细胞的凋亡情况。结果移植后各时间点Brdu标记的神经干细胞数NSCs+EPCs组明显高于NSCs组（P0.05）；NSCs+EPCs组在各时间点的凋亡率明显低于NSCs组（P0.05）；各时间点NSCs+EPCs组血管新生数量明显多于EPCs组、缺血对照组和NSCs组（P0.05）。结论血管内皮祖细胞可以促进移植入缺血再灌注模型大鼠缺血半暗带的神经干细胞增殖，减少其凋亡；2种细胞共移植可以促进缺血半暗带的血管新生。

Abstract

Objective To investigate the effect of endothelial progenitor cells (EPCs) which complexed with neuron stem cells (NSCs) after transplanted into ischemia and reperfusion (I/R) rat model on the proliferation and apoptosis of the NSCs and vasal construction. Methods Totally 150 SD adult male rats were randomly and equally divided into 5 groups

shamoperation group

I/R group

I/R+NSCs group

I/R+EPCs group

and I/R+NSCs+EPCs group. The rats were further divided into 5 subgroups according to the time points of 3

30 and 60 d after transplantation. I/R rat model was established by reversiblyligating middle cerebral artery occlusion. NSCs were derived from the hippocampus of SD rats born in 24 h and identified with Nestin staining. EPCs were obtained from the artery blood of SD rats and verified with immunocytochemical stainings of CD31

CD34 and KDR after culture. The complex of NSCs and EPCs was produced with aid of laminin. Then the complex were transplanted into the ischemia penumbra of corresponding model rat brain. The proliferation and apoptosis of NSCs

and the fomation of new vessels were observed through immunohistochemistry and doublelabeled immunofluorescence staining. Results The proliferation of NSCs was increased

apoptosis cells were decreased and the new vessels were raised in the complex transplantation when compared with single NSCs or EPCs transplantation groups. Conclusion The complex of NSCs and EPCs transplantation improves the proliferation of NSCs

suppresses cell apoptosis and promotes the formation of new vessels.

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