Exogenous CCN1 promotes proliferation and migration of radiation-injured L929 cells

李招权; 万莹铧; 李军; 司维柯; 粟永萍; 杜业军; 潘静

您当前的位置：

首页 >

文章列表页 >

Exogenous CCN1 promotes proliferation and migration of radiation-injured L929 cells

更新时间：2026-01-09

- Exogenous CCN1 promotes proliferation and migration of radiation-injured L929 cells
- Journal of Army Medical University Vol. 32, Issue 11, Pages: 1127-1130(2010)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R818.05;R329.28;R394.2
- Published：2010
- 稿件说明：
移动端阅览
李招权,万莹铧,李军,司维柯,粟永萍,杜业军,潘静.外源性CCN1促进辐射损伤L929细胞的生长和迁移[J].陆军军医大学学报(原第三军医大学学报),2010,32(11):1127-1130.

　Li Zhaoquan,Wan Yinghua,Li Jun,Si Weike,Su Yongping,Du Yejun,Pan Jing.Exogenous CCN1 promotes proliferation and migration of radiation-injured L929 cells[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(11):1127-1130.
李招权,万莹铧,李军,司维柯,粟永萍,杜业军,潘静.外源性CCN1促进辐射损伤L929细胞的生长和迁移[J].陆军军医大学学报(原第三军医大学学报),2010,32(11):1127-1130. DOI：

　Li Zhaoquan,Wan Yinghua,Li Jun,Si Weike,Su Yongping,Du Yejun,Pan Jing.Exogenous CCN1 promotes proliferation and migration of radiation-injured L929 cells[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(11):1127-1130. DOI：

摘要

目的观察外源性CCN1是否促进辐射损伤L929细胞生长和迁移，以探讨CCN1在放创复合伤修复中的作用。方法以4 Gy γ射线辐射L929细胞作为辐射损伤细胞模型，细胞辐射后分别加入2 ml CCN1或RFP条件培养液，37℃，5%CO

培养，作为CCN1组和RFP组；空白条件培养液培养辐射损伤L929细胞作为空白组。各组进行MTT试验、平板克隆形成试验、细胞周期分析和划痕愈合试验。结果与RFP组和空白组相比，CCN1条件培养液处理后，与空白组辐射损伤L929细胞增殖明显增强（P0.05），与RFP组和空白组相比，克隆形成率明显增高[CCN1组(34.4±3.6)%

RFP组(24.5±2.9)%

空白组(29.5±3.5)%

]

（P0.05），培养5 d时CCN1组S期细胞[(37.3±2.3)%

]

比RFP组[(25.2±1.9)%

]

和空白组[(26.9±1.3)%

]

增多（P0.01），细胞迁移能力明显增强划痕愈合试验在1~5 d的愈合宽度/原宽度值与RFP组和空白组相比差异显著（P0.01）。结论外源性CCN1可促进辐射损伤的成纤维细胞生长和迁移，提示CCN1是放创复合伤修复的促进因素之一。

Abstract

Objective To study the role of Cysteine-rich 61 (Cyr61/CNN1) in repair of combined injury by observing whether exogenous CCN1 promotes the proliferation and migration of radiation-injured L929 cells. Methods A radiation model of L929 cells was induced by γ ray at a dose of 4 Gy. The irradiated L929 cells were cultured in a medium containing 2 ml adenovirus plasmids of CCN1 or RFP at 37 ℃ in an atmosphere containing 5%CO

which served as a CCN1 group and a RFP group

respectively. Irradiated L929 cells cultured in a blank

control medium served as a blank control group. Effects of CCN1 on proliferation and migration of radiationinjured L929 cells were detected by MTT assay

plate colony formation assay

cell cycle analysis

and scratch test of wound healing. Results The proliferation and colony formation rates of L929 cells cultured in a medium containing CCN1 were significantly higher than in RFP and control groups [colony formation rate: (34.4±3.6)% vs (24.5±2.9)% and (29.5±3.5)%

P0.05

]

. Five hours after culture

the amount of L929 cells in the S phase was larger in CCN1 group than in RFP and control groups (37.3%±2.3% vs 252%±1.9% and 26.9%±1.3%

P0.01). Scratch test of wound healing showed that the width/thickness ratio of migrated L929 cells in healed wounds was significantly higher in CCN1 group than in RFP and control groups (P0.01). Conclusion Exogenous CCN1 promotes the proliferation and migration of radiation-injured L929 cells

suggesting that CCN1 is one of the stimulating factors for the repair of combined injury.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Expression of β-arrestin1 in oral squamous cell carcinoma and its effect on cell proliferation, migration and invasion

Effect of RNA sensor IFIH1 on proliferation, migration and invasion of HPV positive cervical cancer cells

Anti-gastric cancer effect of a novel small molecule inhibitor 1-3-51 targeting FOXM1 in vitro

LINC00525 targeting miR-338-3p promotes apoptosis and reduces paclitaxel resistance in gastric cancer cells

Sphingosine-1-phosphate type 1 receptor aggravates neointimal hyperplasia after carotid artery injury in mice

Related Author

HAO Xiaohui

CHEN Min

WU Nan

DING Yunshan

ZHU Lifan

DAI Haitao

喻琳

罗莹

Related Institution

School of Medicine， Shihezi University

Department of Stomatology， the First Affiliated Hospital of Shihezi University

Department of Gastroenterology, Qingdao Central Hospital of Rehabilitation University

Endoscopy Center, Qingdao Central Hospital of Rehabilitation University

Department of Pathology， Second Affiliated Hospital， Army Medical University （Third Military Medical University）

⁰