CXCR4 siRNA inhibits growth of human esophageal squamous carcinoma EC-9706 cell xenograft in nude mice

臧文巧; 轩小燕; 杜英; 李倩如; 李 敏; 杨璇

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CXCR4 siRNA inhibits growth of human esophageal squamous carcinoma EC-9706 cell xenograft in nude mice

更新时间：2026-01-09

- CXCR4 siRNA inhibits growth of human esophageal squamous carcinoma EC-9706 cell xenograft in nude mice
- Journal of Army Medical University Vol. 31, Issue 21, Pages: 2140-2142(2009)
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  CLC： R73-354;R73-362;R735.1
- Published：2009
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臧文巧,轩小燕,杜英,李倩如,李敏,杨璇.靶向CXCR4的siRNA对人食管鳞癌EC9706细胞裸鼠移植瘤生长的抑制作用[J].陆军军医大学学报(原第三军医大学学报),2009,31(21):2140-2142.

　ZANG Wen-qiao,XUAN Xiao-yan,DU Ying,LI Qian-ru,LI Min,YANG Xuan.CXCR4 siRNA inhibits growth of human esophageal squamous carcinoma EC-9706 cell xenograft in nude mice[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(21):2140-2142.
臧文巧,轩小燕,杜英,李倩如,李敏,杨璇.靶向CXCR4的siRNA对人食管鳞癌EC9706细胞裸鼠移植瘤生长的抑制作用[J].陆军军医大学学报(原第三军医大学学报),2009,31(21):2140-2142. DOI：

　ZANG Wen-qiao,XUAN Xiao-yan,DU Ying,LI Qian-ru,LI Min,YANG Xuan.CXCR4 siRNA inhibits growth of human esophageal squamous carcinoma EC-9706 cell xenograft in nude mice[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(21):2140-2142. DOI：

摘要

目的探讨CXCR4 siRNA对人食管鳞癌裸鼠移植瘤生长的抑制作用。方法建立人食管鳞癌细胞株EC9706 裸鼠移植瘤动物模型，瘤旁注射化学合成CXCR4 siRNA，同时以瘤旁注射阴性对照siRNA和PBS为对照。监测肿瘤生长变化，测量瘤质量。HE染色观察肿瘤病理学变化，RT-PCR、免疫组化方法检测CXCR4 mRNA和蛋白变化。结果裸鼠体内实验结果显示，与对照组比较，CXCR4 siRNA组肿瘤生长受到明显抑制，瘤质量下降；HE染色显示对照组肿瘤体积大、异形明显；RT-PCR、免疫组化结果表明CXCR4 siRNA处理组CXCR4 mRNA和蛋白表达下调。结论 CXCR4 siRNA能抑制人食管鳞癌EC9706细胞株裸鼠皮下移植瘤的形成，且能在体内有效下调CXCR4 mRNA和蛋白的表达。

Abstract

Objective To investigate the inhibitory effect of CXC chemokine receptor 4 (CXCR4) siRNA on the growth of transplanted tumors of esophageal squamous carcinoma in nude mice. Methods Nude mice model bearing esophageal squamous carcinoma was reproduced by implanting human esophageal squamous carcinoma cell line EC-9706. CXCR4 siRNA was injected to the area nearby the tumors. The tumors injected with control siRNA or PBS served as control. Tumor growth and tumor weight were measured. Histopathological changes of the tumors were observed with HE staining

and the mRNA and protein expressions of CXCR4 were detected by RT-PCR and immunohistochemical staining respectively. Results Tumor growth was obviously suppressed and tumor volume was significantly smaller in the group treated by with CXCR4 siRNA than in control groups. While the histopathological features in the control group showed that the tumor volumes were bigger and large amount of atypic tumor cells was seen. RT-PCR and immunohistochemical staining indicted that CXCR4 mRNA and protein expressions were reduced in in the group treated by with CXCR4 siRNA. Conclusion CXCR4 siRNA inhibits the tumorigenesis of EC9706 cell xenograft in nude mice and down-regulates the CXCR4 mRNA and protein expressions in vivo.

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