Content determination of riparsaponin in Homonoia riparia Lour by HPLC

车仁宇; 赵晶; 王圣燕

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Content determination of riparsaponin in Homonoia riparia Lour by HPLC

更新时间：2026-01-09

- Content determination of riparsaponin in Homonoia riparia Lour by HPLC
- Journal of Army Medical University Vol. 31, Issue 22, Pages: 2275-2277(2009)
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  CLC： R282.71;R284.1
- Published：2009
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车仁宇,赵晶,王圣燕.高效液相法测定水杨柳提取物中水柳皂苷的含量[J].陆军军医大学学报(原第三军医大学学报),2009,31(22):2275-2277.

　CHE Ren-yu,ZHAO Jing,WANG Sheng-yan.Content determination of riparsaponin in Homonoia riparia Lour by HPLC[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(22):2275-2277.
车仁宇,赵晶,王圣燕.高效液相法测定水杨柳提取物中水柳皂苷的含量[J].陆军军医大学学报(原第三军医大学学报),2009,31(22):2275-2277. DOI：

　CHE Ren-yu,ZHAO Jing,WANG Sheng-yan.Content determination of riparsaponin in Homonoia riparia Lour by HPLC[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(22):2275-2277. DOI：

摘要

目的建立测定水杨柳提取物中水柳皂苷的高效液相色谱法。方法采用RP-HPLC法，色谱柱为HYPERSILBDS-C

色谱柱（200 mm×4.6 mm，5 μm）；流动相为乙腈∶水=75∶25；流速为1.0 ml/min；检测波长为203 nm；柱温为30 ℃；柱压为10.0 MPa。结果该方法的线性范围为5～500 μg/ml(r=0.999 8)；平均加样回收率为99.44%，RSD为0.52%（n=15）。结论该方法重现性好，精密度高，可用于水杨柳药材及其制剂的质量控制。

Abstract

Objective To develop a HPLC method for determining the content of riparsaponin in Homonoia riparia Lour. Methods The determination was performed on the HYPERSILBDS-C

(200 mm×4.6 mm

5 μm) with mobile phase consisted of acetonitrile-water (75∶25) at a flow rate of 1.0 ml/min. The detection wavelength was set at 203 nm

the column temperature was 30 ℃， and the column pressure was 10.0 MPa. Results There was a good linear relationship for riparsaponin over the ranges of 5 to 500 μg/ml (r=0.999 8). The average recovery was 99.44%

and RSD was 0.52% (n=15). Conclusion The method has good reproducibility and high precision. It can be applied in quality control of Homonoia riparia Lour.

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