Objective    To analyze the distribution of human papillomavirus (HPV) genotype in the diseases associated with HPV infection and explore the role of HPV genotype in these diseases.     Methods    Retrospective analysis was carried out to compare HPV genotypes of 353 outpatients suffering the diseases associated with HPV infection including cervical caner

cervical intraepithelial neoplasia (CIN)

genital duc condyloma

and subclinical cervical human papillomavirus infection (SPI) in our department from January to October 2008. HybriMax were used to detect HPV genotype. The distribution data of HPV genotype were statistically analyzed.     Results    Totally 176 HPV positive females were found with a positive rate of 49.86%. HPV positive rate in cervical cancer was 100%

and all of them were high risk HPV. The positive rate was increased with the severity of CIN

and the higher histological-grade was

the higher HPV positive rate was (P0.05). Fifteen genotypes were detected by HybriMax. The total HPV genotype were HPV16

HPV 58

HPV 52

HPV 33

HPV 53

HPV 11

HPV 31

HPV 6

HPV 18

HPV 39

HPV 66

HPV 68

HPV 51

HPV 42

and HPV 43 in a decreasing order. The HPV genotypes were differently distributed in various diseases associated with HPV infection. The first 4 genotypes were also coincidence in cervical cancer and CIN. The high risk genotypes HPV16 and HPV18 were the most common genotypes not only in cervical cancer and CIN but also in condyloma and SPI. The low risk genotypes were rare in the benign cervical diseases conversely.     Conclusion    It is necessary to detect HPV genotype because the regional distinctions exist in the prevalence of HPV genotypes in diseases above. HPV16 genotype is the most common genotype that threatens female health. Close attention should be paid in the treatments and following up of benign lesion patients such as genital condylomata and SPI.