Effect of uremic serum on endothelial cell apoptosis and intervention of recombinant human erythropoietin in the process

李小丽; 甘华; 常晓东; 杜晓刚

您当前的位置：

首页 >

文章列表页 >

Effect of uremic serum on endothelial cell apoptosis and intervention of recombinant human erythropoietin in the process

更新时间：2026-01-09

- Effect of uremic serum on endothelial cell apoptosis and intervention of recombinant human erythropoietin in the process
- Journal of Army Medical University Vol. 31, Issue 22, Pages: 2255-2257(2009)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R329.28;R331.1;R692.5
- Published：2009
- 稿件说明：
移动端阅览
李小丽,甘华,常晓东,杜晓刚.尿毒症患者血清对内皮细胞凋亡的影响及rhEPO的干预作用[J].陆军军医大学学报(原第三军医大学学报),2009,31(22):2255-2257.

　LI Xiao-li,GAN Hua,CHANG Xiao-dong,DU Xiao-gang.Effect of uremic serum on endothelial cell apoptosis and intervention of recombinant human erythropoietin in the process[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(22):2255-2257.
李小丽,甘华,常晓东,杜晓刚.尿毒症患者血清对内皮细胞凋亡的影响及rhEPO的干预作用[J].陆军军医大学学报(原第三军医大学学报),2009,31(22):2255-2257. DOI：

　LI Xiao-li,GAN Hua,CHANG Xiao-dong,DU Xiao-gang.Effect of uremic serum on endothelial cell apoptosis and intervention of recombinant human erythropoietin in the process[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(22):2255-2257. DOI：

摘要

目的探讨尿毒症患者血清对人脐静脉内皮细胞(human umbilical vein endothelial cells

HUVECs)凋亡的影响及重组人促红细胞生成素（recombinant human erythropoietin

rhEPO）的干预作用。方法选择我院2008年12月至2009年4月收治的尿毒症患者和健康志愿者各10例，HUVECs按不同的干预方式分3组进行实验：对照组(含10％健康者血清)、尿毒症组（含10％尿毒症患者血清）、rhEPO干预组（rhEPO 5、10、15 U/ml，分别加入含有10％尿毒症患者血清的培养基）。各组干预24 h，TUNEL法检测细胞凋亡，比色法测定细胞内活性氧。结果尿毒症患者血清环境中内皮细胞的凋亡指数和活性氧水平均高于对照组(P0.01)。rhEPO可抑制尿毒症患者血清诱导的内皮细胞凋亡(P0.01)，降低细胞内活性氧水平(P0.01)；随着rhEPO浓度的增加，内皮细胞凋亡指数和ROS水平都逐渐降低(P0.05)。相关分析显示：凋亡指数与活性氧水平呈正相关(r=0.89，P0.01)。结论尿毒症患者血清可以诱导HUVECs凋亡，其机制可能与氧化应激有关。rhEPO可能通过减轻氧化应激反应而抑制尿毒症患者血清诱导的内皮细胞凋亡。

Abstract

Objective To explore the effect of uremic serum on apoptosis in human umbilical vein endothelial cells (HUVECs) and the intervention by recombinant human erythropoietin (rhEPO) in this process. Methods From Dec. 2008 to Apr. 2009

10 uremic patients and 10 healthy volunteers were enrolled in this study. HUVECs were divided into 3 groups: control group (including 10% healthy serum medium)

uremic group (including 10% uremic serum medium) and rhEPO treatment group (rhEPO at 5

10 or 15 U/ml was added to 10% uremic serum medium). After 24 hous’s intervention

cell apoptosis was evaluated by TUNEL assay

and reactive oxygen species were detected by colorimetry. Results HUVECs apoptosis index and ROS level were higher in the presence of uremic serum than those of healthy serum (P0.01). With the addition of rhEPO (5

10 or 15 U/ml) in culture medium

the percentage of apoptotic cells and the production of ROS in HUVECs were inhibited (P0.01). With rhEPO concentration increasing

apoptotic index and ROS level were decreasing gradually (P0.05). Correlation analysis showed that there was positive correlation between apoptotic index and ROS level (r=0.89

P0.01). Conclusion Uremic serum can induce HUVECs apoptosis

and the mechanism may be related to oxidative stress. Recombinant human EPO may inhibit endothelial cell apoptosis induced by uremic serum through alleviating oxidative stress.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Role and mechanism of SIRT3 in oxidative stress and mitochondrial biogenesis in alveolar type II cells

p300 promotes hepatic lipid accumulation in dyslipidemia by regulating SREBP-1c acetylation

Protein phosphatase 2A promotes mitophagy to alleviate fructose-induced mitochondrial oxidative damage in M2-type macrophages

Regulation and mechanism of Gm49394 on islet-β cell apoptosis

Neuroprotective effects and mechanisms of osteocalcin in an Alzheimer’s disease cell model

Related Author

ZHOU Jun

LIU Yaojun

LIU Jing

GAO Ruobing

LI Changhong

FENG Xifeng

SHEN Hao

HUANG Hong

Related Institution

Department of Human Anatomy， Anhui Medical University

Laboratory of Stem Cell and Tissue Engineering， Chongqing Medical University

Institute of Field Surgery， Daping Hospital， Army Medical University

Department of Geriatrics and Special Service Medicine， First Affiliated Hospital（Southwest Hospital）， Army Medical University （Third Military Medical University）

达州市中西医结合医院药学科

⁰