Effects of total alkaloids of Tripterygium hypoglaucum Hutch on proliferation and apoptosis of human colon cancer cell line  HCT116

黄晓春; 刘晋祎; 周燕虹; 敖琳; 杨录军; 曹佳

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Effects of total alkaloids of Tripterygium hypoglaucum Hutch on proliferation and apoptosis of human colon cancer cell line HCT116

更新时间：2026-01-09

- Effects of total alkaloids of Tripterygium hypoglaucum Hutch on proliferation and apoptosis of human colon cancer cell line HCT116
- Journal of Army Medical University Vol. 31, Issue 22, Pages: 2246-2248(2009)
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  CLC： R285.5;R329.28;R735.35
- Published：2009
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黄晓春,刘晋祎,周燕虹,敖琳,杨录军,曹佳.昆明山海棠总生物碱对人结肠癌HCT116细胞增殖和凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(22):2246-2248.

　HUANG Xiao-chun,LIU Jin-yi,ZHOU Yan-hong,AO Lin,YANG Lu-jun,CAO Jia.Effects of total alkaloids of Tripterygium hypoglaucum Hutch on proliferation and apoptosis of human colon cancer cell line HCT116[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(22):2246-2248.
黄晓春,刘晋祎,周燕虹,敖琳,杨录军,曹佳.昆明山海棠总生物碱对人结肠癌HCT116细胞增殖和凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(22):2246-2248. DOI：

　HUANG Xiao-chun,LIU Jin-yi,ZHOU Yan-hong,AO Lin,YANG Lu-jun,CAO Jia.Effects of total alkaloids of Tripterygium hypoglaucum Hutch on proliferation and apoptosis of human colon cancer cell line HCT116[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(22):2246-2248. DOI：

摘要

目的研究昆明山海棠总生物碱(total alkaloids of tripterygium hypoglaucum hutch， THHta)对人结肠癌HCT116细胞增殖及凋亡的影响。方法采用MTT法检测THHta对HCT116细胞增殖的抑制作用；用倒置及荧光显微镜观察THHta作用后HCT116细胞形态的变化；流式细胞术检测THHta对HCT116细胞周期及凋亡的影响，Western bolt检测凋亡相关caspase-3 及PARP蛋白表达。结果 THHta能显著抑制HCT116细胞增殖，抑制率与剂量和时间呈依赖关系。THHta作用HCT116细胞48、72 h的IC

值小于20 μg/ml。THHta（10 μg/ml）能诱导HCT116细胞G

期比例增加及细胞凋亡，caspase-3活化及PARP蛋白剪切显著增强。结论 THHta能显著抑制HCT116细胞增殖，并可诱导HCT116细胞G

期阻滞及细胞凋亡。

Abstract

Objective To investigate the effects of total alkaloids of tripterygium hypoglaucum hutch (THHta) on the proliferation and apoptosis of human colon cancer cell line HCT116. Methods The effects of THHta on the proliferation of HCT116 cells were assessed by MTT assay. Morphological changes of HCT116 cells were observed with invert microscopy and fluorescence microscopy. The cell cycle distribution and apoptosis of HCT116 cells were examined by flow cytometry (FCM). The expression of active caspase-3 and cleaved PARP were detecte

d by Western blot assay. Results THHta inhibited the proliferation of HCT116 cells in a concentration- and time-dependent manner. The IC

values of THHta which affected HCT116 cells for 48 and 72 h were less than 20 μg/ml. THHta (10 μg/ml) increased the percentage of cells at the G

phase

induced the apoptosis of HCT116 cells

and increased the amount of active caspase-3 and cleaved PARP in HCT116 cells. Conclusion THHta inhibits proliferation

causes G

phase arrest and induces apoptosis in cer HCT116 cells in vitro.

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