Antibacterial activity of active component of Crataegus pinnatifida combined with β-Lactam antibiotics against methicillin-resistant Staphylococcus aureus in vitro

姜珂; 覃容欣; 朱仝飞; 周红; 郑江

您当前的位置：

首页 >

文章列表页 >

Antibacterial activity of active component of Crataegus pinnatifida combined with β-Lactam antibiotics against methicillin-resistant Staphylococcus aureus in vitro

更新时间：2026-01-09

- Antibacterial activity of active component of Crataegus pinnatifida combined with β-Lactam antibiotics against methicillin-resistant Staphylococcus aureus in vitro
- Journal of Army Medical University Vol. 31, Issue 19, Pages: 1887-1889(2009)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R285.5;R695;R978.11
- Published：2009
- 稿件说明：
移动端阅览
姜珂,覃容欣,朱仝飞,周红,郑江.山楂活性组分与β-内酰胺类抗生素的联合抑菌作用[J].陆军军医大学学报(原第三军医大学学报),2009,31(19):1887-1889.

　JIANG Ke,QIN Rong-xin,ZHU Tong-fei,ZHOU Hong,ZHENG Jiang.Antibacterial activity of active component of Crataegus pinnatifida combined with β-Lactam antibiotics against methicillin-resistant Staphylococcus aureus in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(19):1887-1889.
姜珂,覃容欣,朱仝飞,周红,郑江.山楂活性组分与β-内酰胺类抗生素的联合抑菌作用[J].陆军军医大学学报(原第三军医大学学报),2009,31(19):1887-1889. DOI：

　JIANG Ke,QIN Rong-xin,ZHU Tong-fei,ZHOU Hong,ZHENG Jiang.Antibacterial activity of active component of Crataegus pinnatifida combined with β-Lactam antibiotics against methicillin-resistant Staphylococcus aureus in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(19):1887-1889. DOI：

摘要

目的研究山楂提取物与β-内酰胺类抗生素对耐甲氧西林金黄色葡萄球菌（methicillin-resistant staphylococcus aureus

MRSA）的体外联合抗菌作用。方法干燥山楂去核切片75％乙醇提取，依次经乙酸乙酯、正丁醇萃取，取正丁醇层经D-101大孔树脂、硅胶柱色谱分离得到组分CP，体外药敏试验验证其联合4种β-内酰胺类抗生素(苯唑西林、舒氨西林、氨苄西林、头孢唑啉)抑制MRSA的作用。结果单独应用苯唑西林、舒氨西林、氨苄西林、头孢唑啉及组分CP对实验株MRSA的最低抑菌浓度(MIC)分别为256、512、512、128、1 024 μg/ml；联合用药时，亚MIC浓度的组分CP(128 μg/ml)使苯唑西林、舒氨西林、氨苄西林、头孢唑啉MIC分别降低至2、32、16、2 μg/ml

部分抑菌指数(FIC)分别为0.13、0.16、0.19、0.14，均小于0.5。结论山楂活性组分CP与β-内酰胺类抗生素具有显著的体外协同抑制MRSA的作用。

Abstract

Objective To study the in vitro antibacterial activities of the Crataegus pinnatifida (C. p) extracts in combination with 4 kinds of β-lactams antibiotics against methicillin-resistant Staphylococcus aureus (MRSA). Methods The dried fruits of C. p were extracted with 75% EtOH followed by being extracted successively with EtOAc and n-BuOH

then the obtained n-BuOH layer was subjected to macroporous D-101 and then to silica gel column chromatography. Finally we obtained the active component of C. p (CP)

and validate the in vitro antibacterial activities of the component CP in combination with 4 kinds of β-lactams antibiotics against MRSA. Results The minimal inhibitory concentration (MIC) of Oxacillin

Ampicillin sodium-sulbactam sodium

Ampicillin

Cephazolin and CP component were 256

512

128 and 1 024 μg/ml

However

when combined with the C. p component at its sub-MIC (128 μg/ml)

the MIC of the above 4 kinds of β-lactams antibiotics was as lower as 2

16 and 2 μg/ml respectively.The fractional inhibitory concentration (FIC) of them were 0.13

0.19

0.16 and 0.14

respectively. Conclusion C. p component has synergistic antibacterial effect on MRSA when combined with β-lactams antibiotics.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Inhibitory effect of emodin on MRSA biofilm and its mechanism

Clinical efficacy of teicoplanin combined with L-carnitine for methicillin-resistant Staphylococcus aureus infective endocarditis

Beta-thujaplicin/ZnO nanoparticles/polycaprolactone/poly(ethylene glycol) nanofibers promotes healing of wounds with methicillin-resistant Staphylococcus aureus infection

Construction of T7 phage display cDNA library from methicillin-resistant Staphylococcus aureus N315

Mechanisms in replacement of major methicillin-resistant Staphylococcus aureus epidemic clones in Chongqing

Related Author

刘明

李海波

刘畅

段浩

蔡仑

刘婧怡

张金盈

王晓莉

Related Institution

No data

⁰