Silencing CXCR7 decreases invasion and adhesion of human breast cancer cells

郑科; 厉红元; 苏新良; 王小毅; 谭金祥; 孔令全; 任国胜

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Silencing CXCR7 decreases invasion and adhesion of human breast cancer cells

更新时间：2026-01-09

- Silencing CXCR7 decreases invasion and adhesion of human breast cancer cells
- Journal of Army Medical University Vol. 32, Issue 1, Pages: 63-66(2010)
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  CLC： R737.9;R394.3;R73-37
- Published：2010
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郑科,厉红元,苏新良,王小毅,谭金祥,孔令全,任国胜.CXCR7/CXCL12对人乳腺癌细胞侵袭和黏附能力的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(01):63-66.

　Zheng Ke,Li Hongyuan,Su Xinliang,Wang Xiaoyi,Tan Jinxiang,Kong Lingquan,Ren Guosheng.Silencing CXCR7 decreases invasion and adhesion of human breast cancer cells[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(01):63-66.
郑科,厉红元,苏新良,王小毅,谭金祥,孔令全,任国胜.CXCR7/CXCL12对人乳腺癌细胞侵袭和黏附能力的影响[J].陆军军医大学学报(原第三军医大学学报),2010,32(01):63-66. DOI：

　Zheng Ke,Li Hongyuan,Su Xinliang,Wang Xiaoyi,Tan Jinxiang,Kong Lingquan,Ren Guosheng.Silencing CXCR7 decreases invasion and adhesion of human breast cancer cells[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(01):63-66. DOI：

摘要

目的探讨趋化因子受体CXCR7及其配体CXCL12对人乳腺癌细胞MDA-MB-435s移动性、侵袭能力以及黏附能力的影响。方法运用RNA干扰技术，沉默人乳腺癌细胞MDA-MB-435s CXCR7基因的表达，分别用RT-PCR、Western blot检测CXCR7 mRNA及蛋白表达水平；划痕实验检测细胞移动性的变化；Transwell小室侵袭实验检测细胞侵袭能力的变化；黏附实验检测肿瘤细胞与细胞外基质的黏附能力。结果有效沉默CXCR7的表达后，与空白对照组比较，CXCR7 mRNA及蛋白表达水平均明显降低（P0.05），CXCL12诱导的人乳腺癌细胞移动速度减慢，侵袭能力和黏附能力明显减弱（P0.05）。结论 CXCR7表达的改变能够调节人乳腺癌细胞移动性、侵袭和黏附能力。CXCL12/CXCR7生物轴在乳腺癌的侵袭转移过程中可能具有重要作用。

Abstract

Objective To explore the effect of chemokine receptor CXCR7 and its ligand CXCL12 on the migration

invasion and adhesion of breast cancer cell line MDA-MB-435s. Methods The expression of the CXCR7 on MDA-MB-435s cells was inhibited by RNA interference. Expression levels of CXCR7 mRNA and CXCR7 protein were assayed by RT-PCR and Western blot respectively. Effect of altered expression of CXCR7 on cell migration was measured by cell scratch assay

cell invasion by Transwell chamber and cell adhesion by adhesion assay respectively. Results The expression levels of CXCR7 mRNA and CXCR7 protein

and the migration

invasion and adhesion of the cancer cells were all decreased significantly after the silencing of CXCR7 compared with the control cells. Conclusion The migration

invasion and adhesion of human breast cancer cells are regulated by CXCR7. CXCL12/CXCR7 axis may play an important role in the invasion and metastasis of breast cancer.

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Related Author

任国胜

孔令全

谭金祥

王小毅

苏新良

厉红元

郑科

TAN Xiaoling

Related Institution

Department of Cold Region Medicine

Key Laboratory of Extreme Environmental Medicine of Ministry of Education

Department of Plateau Physiology and Pathology， Faculty of High Altitude Military Medicine， Army Medical University （Third Military Medical University）

Center for Reproductive Medicine， Chongqing Health Center for Women and Children （Women and Children Hospital of Chongqing Medical University）

Medical School， Hubei Minzu University

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