Berberine hydrochloride inhibits formation of Staphylococcus epidermidis biofilm: relative gene expressions

何敏; 廖璞; 尹一兵; 左帜健

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Berberine hydrochloride inhibits formation of Staphylococcus epidermidis biofilm: relative gene expressions

更新时间：2026-01-09

- Berberine hydrochloride inhibits formation of Staphylococcus epidermidis biofilm: relative gene expressions
- Journal of Army Medical University Vol. 31, Issue 19, Pages: 1872-1875(2009)
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  CLC： R285.5;R378.11;R394.2
- Published：2009
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何敏,廖璞,尹一兵,左帜健.盐酸小檗碱对表皮葡萄球菌生物被膜形成及相关基因表达的抑制作用[J].陆军军医大学学报(原第三军医大学学报),2009,31(19):1872-1875.

　HE Min,LIAO Pu,YIN Yi-bing,ZUO Zhi-jian.Berberine hydrochloride inhibits formation of Staphylococcus epidermidis biofilm: relative gene expressions[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(19):1872-1875.
何敏,廖璞,尹一兵,左帜健.盐酸小檗碱对表皮葡萄球菌生物被膜形成及相关基因表达的抑制作用[J].陆军军医大学学报(原第三军医大学学报),2009,31(19):1872-1875. DOI：

　HE Min,LIAO Pu,YIN Yi-bing,ZUO Zhi-jian.Berberine hydrochloride inhibits formation of Staphylococcus epidermidis biofilm: relative gene expressions[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(19):1872-1875. DOI：

摘要

目的通过检测盐酸小檗碱单用或联合抗菌药物对表皮葡萄球菌（Staphylococcus epidermidis

Se.）形成生物被膜及所需基因的抑制作用。方法体外构建表皮葡萄球菌生物被膜模型，盐酸小檗碱单用或联合万古霉素、环丙沙星、红霉素分别干预生物被膜的形成，采用CRA平板法检测药物对Se.生物被膜的抑制作用，荧光定量RT-PCR方法检测药物作用前后表皮葡萄球菌icaA基因和agr基因的表达变化。结果盐酸小檗碱对icaA基因和agr基因的影响作用不及万古霉素、环丙沙星、红霉素；当不同浓度的盐酸小檗碱分别与万古霉素、环丙沙星、红霉素联用时，随着盐酸小檗碱的浓度增加，联合用药对icaA基因和agr基因的抑制作用逐渐减弱。结论中药单体（盐酸小檗碱）对Se.形成生物被膜及所需关键基因的表达有一定的抑制作用，但抑制作用不及抗生素（万古霉素、环丙沙星、红霉素），并且不能与抗生素（万古霉素、环丙沙星、红霉素）联合用药，它们之间存在拮抗作用。

Abstract

Objective To investigate the inhibitory effect of Berberine hydrochloride alone or combined with antibiotics on the formation of Staphylococcus epidermidis biofilm and the expressions of relative genes in order to explore a new way to treat the infection induced by the bacteria. Methods Biofilm of Staphylococcus epidermidis was constructed in vitro first

and then Berberine hydrochloride alone or combined with norvancomycin

ciprofloxacin or erythromycin was used to affect the biofilm formation. Then the inhibition was detected by CRA

and the expressions of icaA and agr by the way of fluorescent quantitation RT-PCR. Results The effectiveness of Berberine hydrochloride on the expressions of icaA and agr in Staphylococcus epidermidis was not as better as the other antibiotics. When different-dosed berberine hydrochloride was combined with norvancomycin

ciprofloxacin or erythromycin to affect the biofilm

the inhibition on the expressions of icaA and agr showed a negatively dose-dependent fashion. Conclusion Berberine hydrochloride can inhibit the formation of the biofilm of Staphylococcus epidermidisthrough suppressing the relative genes

though not as good as the antibiotics

such as norvancomycin

ciprofloxacin and erythromycin. It should not be combined with these antibiotics because the effectiveness is antagonism.

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