Relationship of telomerase activity with proliferation, migration and adhering of EPCs in peripheral blood in vitro

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Relationship of telomerase activity with proliferation, migration and adhering of EPCs in peripheral blood in vitro

更新时间：2026-01-09

- Relationship of telomerase activity with proliferation, migration and adhering of EPCs in peripheral blood in vitro
- Journal of Army Medical University Vol. 31, Issue 20, Pages: 1986-1988(2009)
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  CLC： R322.12;R329.25;R329.28
- Published：2009
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韩娟萍,哈小琴,吕同德,张卫泽,尹强.内皮祖细胞增殖、迁移和黏附能力与端粒酶活性的关系[J].陆军军医大学学报(原第三军医大学学报),2009,31(20):1986-1988.

　HAN Juan-ping,HA Xiao-qin,LU Tong-de,ZHANG Wei-ze,YIN Qiang.Relationship of telomerase activity with proliferation, migration and adhering of EPCs in peripheral blood in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(20):1986-1988.
韩娟萍,哈小琴,吕同德,张卫泽,尹强.内皮祖细胞增殖、迁移和黏附能力与端粒酶活性的关系[J].陆军军医大学学报(原第三军医大学学报),2009,31(20):1986-1988. DOI：

　HAN Juan-ping,HA Xiao-qin,LU Tong-de,ZHANG Wei-ze,YIN Qiang.Relationship of telomerase activity with proliferation, migration and adhering of EPCs in peripheral blood in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(20):1986-1988. DOI：

摘要

目的观察体外培养外周血内皮祖细胞(EPCs) 增殖、迁移和黏附能力与EPCs端粒酶活性之间的关系。方法密度梯度离心法获取人外周血单个核细胞(MNCs)，接种至明胶包被的培养板上

培养7 d后进行细胞免疫组化鉴定EPCs，采用MTT比色法、Transwell小室和黏附能力测定实验

观察EPCs的增殖、迁移和黏附能力。TRAP-PCR银染法检测EPCs端粒酶活性。结果从人外周血能成功分离EPCs细胞。从冠心病患者分离的EPCs增殖、迁移、黏附能力较非冠心病患者下降。冠心病患者EPCs端粒酶活性较非冠心病患者低。结论冠心病患者EPCs功能显著减弱

可能与EPCs端粒酶活性降低有关。

Abstract

Objective To investigate the relationship of the activity of telomerase with the proliferation

migration and adhering of cultured endothelial progenitor cells (EPCs). Methods Mononuclear cells (MNCs) were harvested from human peripheral blood by density gradient centrifugation

then inoculated in culture plates coated with gelatin. After cultured for 7 d

the immunohistochemical method was performed to identify EPCs. The proliferation

migration and adhering of EPCs were measured by MTT assay

Transwell insert and the measured experiment of adhering ability. The activity of telomerase was detected by TRAP-PCR argentation. Results EPCs were well isolated from human peripheral blood. The proliferation

migration and adhering of EPCs and the activity of telomerase were reduced in patients with coronary heart disease (CHD)

compared with the control group. Conclusion The weakening of EPCs biological function might be correlated with the attenuation of telomerase activity.

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Related Institution

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