Localization of transplanted UC-MSCs in mice with premature ovarian failure

李彩霞; 王凤英; 冷少华; 梁志清; 李玉艳

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Localization of transplanted UC-MSCs in mice with premature ovarian failure

更新时间：2026-01-09

- Localization of transplanted UC-MSCs in mice with premature ovarian failure
- Journal of Army Medical University Vol. 32, Issue 1, Pages: 57-59(2010)
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  CLC： R711.75;R329.2;R-332
- Published：2010
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李彩霞,王凤英,冷少华,梁志清,李玉艳.脐带基质干细胞移植在卵巢早衰模型小鼠体内的分布[J].陆军军医大学学报(原第三军医大学学报),2010,32(01):57-59.

　Li Caixia,Wang Fengying,Leng Shaohua,Liang Zhiqing,Li Yuyan.Localization of transplanted UC-MSCs in mice with premature ovarian failure[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(01):57-59.
李彩霞,王凤英,冷少华,梁志清,李玉艳.脐带基质干细胞移植在卵巢早衰模型小鼠体内的分布[J].陆军军医大学学报(原第三军医大学学报),2010,32(01):57-59. DOI：

　Li Caixia,Wang Fengying,Leng Shaohua,Liang Zhiqing,Li Yuyan.Localization of transplanted UC-MSCs in mice with premature ovarian failure[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(01):57-59. DOI：

摘要

目的观察人脐带基质干细胞（umbilical cord-matrix stem cells

UC-MSCs）移植后在卵巢早衰（premature ovarian failure

POF）小鼠模型中的分布与生长情况。方法用环磷酰胺（cyclophosphamide

CPA）和白消安(busulfan

BUS)处理，建立小鼠POF模型；分离扩增UCMSCs，采用腺病毒介导Ad-GFP转染方式对其进行标记；将GFP阳性细胞移植入POF小鼠体内，分别于移植后1、10、20 d留取组织，通过激光共聚焦显微镜观察UC-MSCs在小鼠卵巢及其他组织中的定位与生长情况。结果腺病毒介导Ad-GFP转染UC-MSCs效率达95%左右。POF小鼠经尾静脉接受UC-MSCs移植后1 d，卵巢可见GFP阳性细胞，移植后10 d及20 d的卵巢GFP阳性细胞明显增加，而小鼠骨髓、大脑、肝脏、肾脏、胰腺等组织无明显GFP阳性细胞存在。结论人UC-MSCs经尾静脉移植可向POF小鼠卵巢归巢，并能在局部存活生长，提示其可能具有卵巢损伤修复的作用。

Abstract

Objective To observe the localization of transplanted human umbilical cord-matrix stem cells (UC-MSCs) in mouse premature ovarian failure (POF) model. Methods POF animal model was established by cyclophosphamide (CPA) and busulfan (BUS) chemotherapy. After UC-MSCs were isolated and proliferated from UC

recombinant Ad-enovirus-green fluorescent protein (Ad-GFP) was transfected in them via Ad-enovirus. The GFP-transfected UC-MSCs (2×10

to 3×10

) were transplanted into POF mice through caudal vein

and tissues from POF mice were obtained for frozen section at 1

10 and 20 d after

transplantation. GFP-positive cells in the ovary

brain

liver

kidney and pancreas were determined by laser confocal microscopy. Results Transfection efficiency of Ad-GFP by Ad-enovirus was about 95%. After transplantation

GFP-positive cells were found in the harmed ovaries and more GFP-positive cells were found at 10 and 20 d after transplantation than those at 1 d after transplantation. However

no GFP-positive cells were found in the bone marrow

brain

liver

kidney and pancreas. Conclusion Human UC-MSCs can home and survive in the ovaries of POF mouse model. It suggests that UC-MSCs maybe have the potential for POF therapy.

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