Regulation of N-Acetyl-D-glucosamine on macrophages function of stressed mice

裴轶劲; 吴小兰; 毛方圆; 陈婉燕; 刘俊康

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Regulation of N-Acetyl-D-glucosamine on macrophages function of stressed mice

更新时间：2026-01-09

- Regulation of N-Acetyl-D-glucosamine on macrophages function of stressed mice
- Journal of Army Medical University Vol. 31, Issue 19, Pages: 1869-1871(2009)
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  CLC： R-332;R392.12;R977.6
- Published：2009
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裴轶劲,吴小兰,毛方圆,陈婉燕,刘俊康.N-乙酰氨基葡萄糖对应激小鼠巨噬细胞吞噬功能的调节作用[J].陆军军医大学学报(原第三军医大学学报),2009,31(19):1869-1871.

　PEI Yi-jin,WU Xiao-lan,MAO Fang-yuan,CHEN Wan-yan,LIU Jun-kang.Regulation of N-Acetyl-D-glucosamine on macrophages function of stressed mice[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(19):1869-1871.
裴轶劲,吴小兰,毛方圆,陈婉燕,刘俊康.N-乙酰氨基葡萄糖对应激小鼠巨噬细胞吞噬功能的调节作用[J].陆军军医大学学报(原第三军医大学学报),2009,31(19):1869-1871. DOI：

　PEI Yi-jin,WU Xiao-lan,MAO Fang-yuan,CHEN Wan-yan,LIU Jun-kang.Regulation of N-Acetyl-D-glucosamine on macrophages function of stressed mice[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(19):1869-1871. DOI：

摘要

目的探讨N-乙酰氨基葡萄糖（N-Acetyl-D-glucosamine

GlcNAc）对应激小鼠模型腹腔巨噬细胞(peritoneal macrophage

PMφ)吞噬功能的调节作用。方法以足底电刺激法复制应激小鼠模型，将50只小鼠分为正常对照组、应激模型组、低剂量GlcNAc治疗组、中剂量GlcNAc治疗组和高剂量GlcNAc治疗组，每组10只。采用酵母吞噬法检测PMφ吞噬功能，MTT法检测PMφ能量代谢水平，使用原子力显微镜观察PMφ吞噬酵母的情况。结果与正常对照组相比，应激模型组PMφ吞噬功能明显下降(P0.01)，能量代谢水平明显下降(P0.01)，而中剂量和高剂量GlcNAc能有效提高巨噬细胞吞噬功能和调节细胞活性。结论 GlcNAc可促进应激小鼠PMφ的吞噬而改善免疫功能。

Abstract

Objective To investigate the regulative role of N-Acetyl-D-glucosamine (GlcNAc) on the stressed mice macrophages function. Methods The stressed mice model was established by electric footshock method. The mice were divided into 5 groups: normal control group

stressed mice model group

low dose GlcNAc treatment group (0.25 ml 15%GlcNAc)

medium-dose GlcNAc treatment group (0.5 ml 15%GlcNAc) and high-dose GlcNAc treatment group (1 ml 15%GlcNAc). GlcNAc was intragastrically injected to corresponding mice 2 h before the electrical stimulation. Peritoneal macrophage (PMφ) phagocytosis capability was detected by phagocytosis saccharomycete assay

and PMφ energy metabolism was detected by MTT assay. Results Compared with normal control group

stressed mice PMφ phagocytosis capability was significantly lower (P0.01)

and energy metabolism significantly reduced (P0.01). High-dose GlcNAc and medium-dose GlcNAc enhanced effectively PMφ phagocytosis capability and improved the energy metabolism. Conclusion GlcNAc has regulative role on the dysfunction of stressed mice PMφ.

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