Preparation and performance test for AEAPS/Dextran-modified superparamagnetic iron oxide nanoparticles

邓均; 贺娟; 郑峻松; 贾延辉; 黄辉; 方立超; 李艳; 蒋丽莉; 李平

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Preparation and performance test for AEAPS/Dextran-modified superparamagnetic iron oxide nanoparticles

更新时间：2026-01-09

- Preparation and performance test for AEAPS/Dextran-modified superparamagnetic iron oxide nanoparticles
- Journal of Army Medical University Vol. 31, Issue 19, Pages: 1866-1868(2009)
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  CLC： R913;R943;R981
- Published：2009
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邓均,贺娟,郑峻松,贾延辉,黄辉,方立超,李艳,蒋丽莉,李平.AEAPS与葡聚糖共修饰超顺磁纳米颗粒的制备及性质[J].陆军军医大学学报(原第三军医大学学报),2009,31(19):1866-1868.

　DENG Jun,HE Juan,ZHENG Jun-song,JIA Yan-hui,HUANG Hui,FANG Li-chao,LI Yan,JIANG Li-li,LI Ping.Preparation and performance test for AEAPS/Dextran-modified superparamagnetic iron oxide nanoparticles[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(19):1866-1868.
邓均,贺娟,郑峻松,贾延辉,黄辉,方立超,李艳,蒋丽莉,李平.AEAPS与葡聚糖共修饰超顺磁纳米颗粒的制备及性质[J].陆军军医大学学报(原第三军医大学学报),2009,31(19):1866-1868. DOI：

　DENG Jun,HE Juan,ZHENG Jun-song,JIA Yan-hui,HUANG Hui,FANG Li-chao,LI Yan,JIANG Li-li,LI Ping.Preparation and performance test for AEAPS/Dextran-modified superparamagnetic iron oxide nanoparticles[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(19):1866-1868. DOI：

摘要

目的探讨以AEAPS及葡聚糖共修饰超顺磁纳米颗粒（superparamagnetic nanoparticles，SPION）的制备方法

并检测其物理和磁学性质。方法共沉淀一步法制备葡聚糖包被的四氧化三铁纳米颗粒，然后在通氮、60 ℃、强力机械搅拌的条件下加入AEAPS，得到AEAPS与葡聚糖共修饰的SPION。采用透射电镜测量其大小及分布，用振动样品磁强计检测磁化率等参数，用普鲁士蓝染色的方法判断AEAPS/葡聚糖-SPION是否与抗体连接。结果所得样品核心为四氧化三铁晶体，核心粒径不超过20 nm，包被葡聚糖后整体颗粒直径不超过25 nm，质量饱和磁场强度为67.364 88 emu/g Fe，AEAPS/ 葡聚糖-SPION与抗体结合，可对特异性抗原产生抗原-抗体结合，普鲁士蓝染色结果为阳性。结论制备的样品具有粒径小，分散性好，超顺磁性等优点，能与单克隆抗体有效结合。

Abstract

Objective To explore how to prepare N-(2-aminoethyl)-3-aminopropyltrimethoxysilance (AEAPS)-Dextran-modified superparamagnetic iron oxide nanoparticles (SPION) and test the physical and magnetic properties of the nanoparticles. Methods Dextran-modified SPION was obtained by "one step" co-precipitation method. Then

AEAPS was added to the resultant Dextran-SPION under the condition of N2 saturation and high-speed mechanical stirring at 60℃. The size and magnetic susceptibility of the nanoparticles were measured with the aid of transmission electron microscope and Vibrating Sample Magnetometer

respectively. Results The AEAPS-Dextran-modified SPION nanoparticles had core-shell diameter less than 25 nm with magnetic core of less than 20 nm in diameter. The mass saturation magnetic intensity was 65.82319 emu/g Fe. Antibody conjugated with AEAPS-Dextran-modified SPION could bind to its specific antigen and were positive to Prussian Blue staining. Conclusion The AEAPS-Dextran-modified SPION possess the characteristics of small size and good dispersion super-paramagnetic response

and could be to bind to monoclonal antibody for possible application in disease diagnosis and treatment.

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