Preparation and identification of monoclonal antibody against advanced oxidation protein products

田梅; 易维京; 吴雄飞; 余荣杰

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Preparation and identification of monoclonal antibody against advanced oxidation protein products

更新时间：2026-01-09

- Preparation and identification of monoclonal antibody against advanced oxidation protein products
- Journal of Army Medical University Vol. 31, Issue 20, Pages: 1973-1976(2009)
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  CLC： R392-33;R392.11;R392.7
- Published：2009
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田梅,易维京,吴雄飞,余荣杰.晚期氧化蛋白产物抗体的制备和鉴定[J].陆军军医大学学报(原第三军医大学学报),2009,31(20):1973-1976.

　TIAN Mei,YI Wei-jing,WU Xiong-fei,YU Rong-jie.Preparation and identification of monoclonal antibody against advanced oxidation protein products[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(20):1973-1976.
田梅,易维京,吴雄飞,余荣杰.晚期氧化蛋白产物抗体的制备和鉴定[J].陆军军医大学学报(原第三军医大学学报),2009,31(20):1973-1976. DOI：

　TIAN Mei,YI Wei-jing,WU Xiong-fei,YU Rong-jie.Preparation and identification of monoclonal antibody against advanced oxidation protein products[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(20):1973-1976. DOI：

摘要

目的制备和鉴定抗人晚期氧化蛋白产物（AOPPs）单克隆抗体(McAb)。方法用次氯酸（HOCl）和纯化的人血白蛋白(HSA)孵育获得AOPPs-HSA，取凝胶层析纯化后的第1峰免疫BALB /c小鼠，采用杂交瘤技术制备抗 AOPPs-HSA 的 McAb，用间接 ELISA、Western blot鉴定其特异性及亚类。结果成功筛选出3株稳定分泌抗 AOPPs，1株既抗HSA又抗AOPP的McAb杂交瘤细胞株，其分泌抗体亚类3株为IgG1，1株为IgG2b，间接ELISA和Western blot证明制备的抗体可以特异地和天然或体外制备的AOPPs结合。结论获得抗人AOPPs-HSA的 McAb细胞株4株，为进一步研究AOPPs生物学活性及其相关疾病的诊断和预后奠定基础。

Abstract

Objective To prepare and identify the monoclonal antibodies (McAb) against advanced oxidation protein products (AOPPs). Methods BALB/c mice were immunized with purified AOPPs-human serum albumin (HAS)

and then McAbs against AOPPs-HSA was produced by hybridoma technique. Indirect ELISA and Western blot analysis were used to identify the immunoglobin specificity and subtype. Results Three novel McAbs only against AOPPs-HSA and one against both AOPPs-HSA and HSA were harvested. The subclass of 3 McAbs were determined as IgG1 and 1 McAb as IgG2b. McAbs reacted strongly and specifically to protein of nature and HOCL-treated AOPPs by Western blot analysis. Conclusion The 4 McAbs against AOPPs are prepared and identified successfully

which provide an important tool for biological properties of AOPPs and for its detection clinical laboratory.

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