Phylogenetic analysis based on VP1 gene of coxsackievirus A16 strains isolated in Beijing from 2007 to 2008

王娟; 罗珍; 范东瀛; 高娜; 朱俊萍; 安静

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Phylogenetic analysis based on VP1 gene of coxsackievirus A16 strains isolated in Beijing from 2007 to 2008

更新时间：2026-01-09

- Phylogenetic analysis based on VP1 gene of coxsackievirus A16 strains isolated in Beijing from 2007 to 2008
- Journal of Army Medical University Vol. 31, Issue 23, Pages: 2342-2346(2009)
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  CLC： R181.34;R373.23;R394.3
- Published：2009
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王娟,罗珍,范东瀛,高娜,朱俊萍,安静.2007-2008年北京地区CA16VP1区系统进化分析[J].陆军军医大学学报(原第三军医大学学报),2009,31(23):2342-2346.

　WANG Juan,LUO Zhen,FAN Dong-ying,GAO Na,ZHU Jun-ping,AN Jing.Phylogenetic analysis based on VP1 gene of coxsackievirus A16 strains isolated in Beijing from 2007 to 2008[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(23):2342-2346.
王娟,罗珍,范东瀛,高娜,朱俊萍,安静.2007-2008年北京地区CA16VP1区系统进化分析[J].陆军军医大学学报(原第三军医大学学报),2009,31(23):2342-2346. DOI：

　WANG Juan,LUO Zhen,FAN Dong-ying,GAO Na,ZHU Jun-ping,AN Jing.Phylogenetic analysis based on VP1 gene of coxsackievirus A16 strains isolated in Beijing from 2007 to 2008[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(23):2342-2346. DOI：

摘要

目的了解2007-2008年北京地区柯萨奇病毒A组16型（Coxsackievirus A16，CA16）流行情况及其种系进化规律和基因分型。方法收集北京地区2007-2008年133例手足口病病例临床标本，从中筛选CA16毒株，扩增部分毒株VP1区并进行序列测定和分析。结果共筛选得到39例45份CA16阳性标本，测定其中16株病毒的VP1区核苷酸序列，其同源性为91.43%～98.65%，氨基酸同源性为97.98%～100%。该16株病毒与GenBank中选取的24株CA16参考株的核苷酸同源性为78.13%～98.65%，氨基酸同源性为89.9%～100%。16株北京分离株中的9株与近年多数中国大陆分离株形成进化树上亲缘关系较近的一群，其余7株与我国台湾及周边国家的一系列分离株亲缘关系较近。结论 CA16为北京地区手足口病的主要致病原之一，本地区毒株同源性较高，同GenBank中24株CA16参比毒株序列进行系统进化分析显示其属于B基因群，16株北京分离株在系统进化树上有分别归属于两支的趋势。

Abstract

Objective To investigate the epidemic situation and the phylogenetic relationship of Coxsackievirus A16 (CA16) in Beijing from 2007 to 2008. Methods CA16 strains were isolated from the clinical specimens of 133 patients with hand-foot-mouth disease (HFMD). VP1 gene fragments were amplified by RT-PCR. The produced fragments were sequenced and analyzed. Results Totally 45 CA16 positive specimens were identified from 39 patients and the entire VP1 genes of 16 CA16 strains were sequenced. For these 16 strains

the nucleotide identity of VP1 genes ranged from 91.43% to 98.65%

the amino acid identity ranged from 97.98% to 100%. The nucleotide and amino acid identity of VP1 genes between these 16 strains and CA16 reference strains from GenBank was 78.13% to 98.65% and 89.9% to 100% respectively. Nine out of the 16 CA16 strains are closely related with some mainland Chinese isolates in recent years. The other 7 strains are closely related with Taiwan-98 isolate

shzh01-69 isolate and some isolates from other countries. Conclusion CA16 is one of the important pathogens of HFMD in Beijing

and the isolates in this study have close association in genetic relationship. Phylogenetic analysis shows that all of the 16 CA16 strains isolated in this study belong to genogroup B and they might be divided into 2 branches.

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