Cloning of mouse monocyte chemoattractant protein-1 promoter and down-regulation of its activity by farnesoid X receptor

彭家和; 刘红; 张迁; 申丽丽; 张艳; 董金瑜; 苏畅; 江渝

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Cloning of mouse monocyte chemoattractant protein-1 promoter and down-regulation of its activity by farnesoid X receptor

更新时间：2026-01-09

- Cloning of mouse monocyte chemoattractant protein-1 promoter and down-regulation of its activity by farnesoid X receptor
- Journal of Army Medical University Vol. 32, Issue 1, Pages: 33-35(2010)
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  CLC： R392-33;R392.11;R392.2
- Published：2010
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彭家和,刘红,张迁,申丽丽,张艳,董金瑜,苏畅,江渝.小鼠MCP-1基因启动子的克隆及核受体FXR下调其活性初步分析[J].陆军军医大学学报(原第三军医大学学报),2010,32(01):33-35.

　Peng Jiahe,Liu Hong,Zhang Qian,Shen Lili,Zhang Yan,Dong Jinyu,Su Chang,Jiang Yu.Cloning of mouse monocyte chemoattractant protein-1 promoter and down-regulation of its activity by farnesoid X receptor[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(01):33-35.
彭家和,刘红,张迁,申丽丽,张艳,董金瑜,苏畅,江渝.小鼠MCP-1基因启动子的克隆及核受体FXR下调其活性初步分析[J].陆军军医大学学报(原第三军医大学学报),2010,32(01):33-35. DOI：

　Peng Jiahe,Liu Hong,Zhang Qian,Shen Lili,Zhang Yan,Dong Jinyu,Su Chang,Jiang Yu.Cloning of mouse monocyte chemoattractant protein-1 promoter and down-regulation of its activity by farnesoid X receptor[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(01):33-35. DOI：

摘要

目的小鼠单核细胞趋化蛋白-1（monocyte chemoattractant protein-1， MCP-1）启动子的克隆及其活性分析。方法从小鼠巨噬细胞株ANA-1中分别扩增一长一短2个小鼠MCP-1启动子片段，并克隆入虫荧光素酶报告基因载体中。2个不同长度的MCP-1启动子片段重组报告基因质粒分别与类法尼醇X受体(farnesoid X receptor

FXR)真核表达质粒共转染HEK293细胞中，经鹅脱氧胆汁酸(chenodeoxycholic acid

CDCA)处理后，检测荧光素酶活性。结果小鼠MCP-1启动子报告基因载体构建成功。经CDCA处理后，转染FXR表达质粒可下调2个不同长度的MCP-1启动子活性，且下调幅度相当。结论激活FXR可能通过小鼠MCP-1基因启动子-322～+82 bp区域FXR负性结合元件而下调该基因转录活性。

Abstract

Objective To clone the mouse monocyte chemoattractant protein-1 (MCP-1) promoter and analyze its activity. Methods Two promoter fragments of mouse MCP-1 gene were amplified from murine macrophage cell line ANA-1 and then cloned into luciferase reporter vector. They were respectively transfected with or without farnesoid X receptor (FXR) expression vector into HEK293 cells. The luciferase activity of transfected HEK293 after the treatment of chenodeoxycholic acid (CDCA

50 μmol/L) was determined with chemiluminescent analysis and normalized against β-galactosidase activity. Results The 2 luciferase reporter vectors of MCP-1 promoter were successfully constructed. After treatment with CDCA

FXR expression vector transfection equivalently down-regulated the promoter activity of both mouse MCP-1 promoter fragments in HEK293 cells. Conclusion Activated by CDCA

FXR may down-regulate the activity of mouse MCP-1 promoter through negative FXR element which lies between position -322 and +82 bp.

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