Regulation of Delta1 expression in DPSCs by specific siRNA lentivirus vector

王雪飞; 张纲; 裘松波; 何飞; 谭颖徽; 汪蕾; 吴小波

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Regulation of Delta1 expression in DPSCs by specific siRNA lentivirus vector

更新时间：2026-01-09

- Regulation of Delta1 expression in DPSCs by specific siRNA lentivirus vector
- Journal of Army Medical University Vol. 32, Issue 1, Pages: 30-32(2010)
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  CLC： R322.41;R329.24;R394.1
- Published：2010
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王雪飞,张纲,裘松波,何飞,谭颖徽,汪蕾,吴小波.构建siRNA慢病毒载体调控人牙髓干细胞Delta1表达的实验研究[J].陆军军医大学学报(原第三军医大学学报),2010,32(01):30-32.

　Wang Xuefei,Zhang Gang,Qiu Songbo,He Fei,Tan Yinghui,Wang Lei,Wu Xiaobo.Regulation of Delta1 expression in DPSCs by specific siRNA lentivirus vector[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(01):30-32.
王雪飞,张纲,裘松波,何飞,谭颖徽,汪蕾,吴小波.构建siRNA慢病毒载体调控人牙髓干细胞Delta1表达的实验研究[J].陆军军医大学学报(原第三军医大学学报),2010,32(01):30-32. DOI：

　Wang Xuefei,Zhang Gang,Qiu Songbo,He Fei,Tan Yinghui,Wang Lei,Wu Xiaobo.Regulation of Delta1 expression in DPSCs by specific siRNA lentivirus vector[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(01):30-32. DOI：

摘要

目的构建 Notch配体 Delta1基因慢病毒干扰载体及建立 Delta1基因稳定干扰的人牙髓干细胞系。方法将 Delta1基因特异性 siRNA靶序列与双酶切慢病毒载体 pGCSIL-GFP连接，转化，挑取阳性克隆进行 PCR鉴定；利用包装细胞 293T获得重组的慢病毒

感染人牙髓干细胞，分为DPSCs/Delta1-RNAi组、DPSCs/vector组和 DPSCs/wt组；RT-PCR和 Western blot分别检测 Delta1 mRNA及蛋白的表达。结果经 PCR和 DNA测序鉴定，成功构建 Delta1特异性 siRNA的慢病毒载体，并感染牙髓干细胞；经检测 DPSCs/Delta1-RNAi组Delta1 mRNA及蛋白表达较 DPSCs/vector组和 DPSCs/wt组明显降低，DPSCs/vector组和 DPSCs/wt组之间无明显差异。结论通过成功构建的Delta1特异性siRNA 慢病毒载体对人牙髓干细胞的转染实现了对其 Delta1 mRNA和蛋白的调控。

Abstract

Objective To construct a lentivirus vector for RNA interference (RNAi) of Delta1 gene and establish human dental pulp stem cells (DPSCs) with stably inhibited Delta1. Methods Delta1 specific siRNA was ligated with double digested lentivirus vector pGCSIL-GFP and the positive colonies were obtained by PCR

DPSCs were interfered and divided into DPSCs/Delta1-RNAi group

DPSCs/vector group and DPSCs/wt group

and the expression of Delta1 was verified by RT-PCR and Western blot analysis. Results The ligation of Delta1 specific siRNA to the double digested lentivirus vector pGCSIL-GFP was successful. Delta1 protein and Delta1 mRNA expression of DPSCs/Delta1-RNAi group was significantly higher than the DPSCs/vector group and DPSCs/wt group

but there was no different between the DPSCs/vector group and DPSCs/wt group. Conclusion The construction of Delta1 specific siRNA lentivirus vectors is successful and it regulates the expression of Delta1 in DPSCs

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