Silencing survivin gene with RNAi inhibits proliferation of human renal cancer cell line 786-O

李戈; 王明

您当前的位置：

首页 >

文章列表页 >

Silencing survivin gene with RNAi inhibits proliferation of human renal cancer cell line 786-O

更新时间：2026-01-09

- Silencing survivin gene with RNAi inhibits proliferation of human renal cancer cell line 786-O
- Journal of Army Medical University Vol. 31, Issue 24, Pages: 2444-2447(2009)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R394-33;R73-362;R737.11
- Published：2009
- 稿件说明：
移动端阅览
李戈,王明.应用RNAi技术沉默survivin基因对肾癌786-O细胞的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(24):2444-2447.

　LI Ge,WANG Ming.Silencing survivin gene with RNAi inhibits proliferation of human renal cancer cell line 786-O[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(24):2444-2447.
李戈,王明.应用RNAi技术沉默survivin基因对肾癌786-O细胞的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(24):2444-2447. DOI：

　LI Ge,WANG Ming.Silencing survivin gene with RNAi inhibits proliferation of human renal cancer cell line 786-O[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(24):2444-2447. DOI：

摘要

目的运用RNA干扰(RNA interference，RNAi)技术特异性阻断肾癌786-O细胞系中survivin基因的表达，并研究survivin沉默后对786-O细胞的影响。方法用真核转录载体pcDNA

6.2-GW/EmGFPmiR构建针对survivin基因的干扰载体，用脂质体法转染肾癌786-O细胞，通过RT-PCR、免疫组化分别检测survivin基因的mRNA和蛋白水平的表达，MTT法检测细胞生长的影响。结果干扰载体有效地阻断了786-O细胞中survivin基因在mRNA与蛋白水平上的表达，细胞活性受到抑制。结论 RNAi技术沉默survivin基因可以下调肾癌786-O细胞survivin基因的表达，抑制细胞的活性。

Abstract

Objective To explore the inhibitory effects of RNAi targeting survivin to suppress the expression of survivin and to induce the apoptosis of human renal cancer cell line 786-O. Methods The target gene fragments were cloned into pcDNA

6.2-GW/EmGFPmiR vectors

and then the recombinant eukaryotic expression vectors were transfected into 786-O cells with liposomes. The expression changes in survivin mRNA and protein were detected by RT-PCR and immunohistochemical assay

and the proliferation inhibitory rate (IR) of 786-O cells was detected by M’TT assay. Results The mRNA and protein expressions of survivin in the siRNA group were significantly inhibited by the eukaryotic express

ion vectors

and it also inhibited significantly the growth and proliferation of 786-O cells. Conclusion Silencing survivin gene by the RNAi technique can not only effectively decrease the expressions of survivin gene and protein

but also inhibit the cell proliferation in 786-O cells. This may provide the experimental evidence for the survivin gene as a target of gene therapy in renal cancer.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Role of methyl-CpG-binding domain protein 2 in regulating aquaporin-5 expression in human submandibular gland cells in vitro

Effect of LncRNA LINC01140 on proliferation and cell cycle in acute myeloid leukemia U937 cells

Sperm-associated antigen 6, independent on P53, regulates cell apoptosis of myelodysplastic syndromes induced by TRAIL

Vitamin E-coupled nanoparticles-delivered siRNA inhibits expression of hepatitis C virus core protein in vitro

Effect of cyclophilin A down-regulation on growth and metastasis of laryngeal squamous cell carcinoma in nude mice

Related Author

陈中林

聂敏海

贾颖

叶艳艳

邓舒婷

黄葳

刘旭倩

骆青松

Related Institution

No data

⁰