Cyclic strain induces dermal fibroblasts orientation through integrin β<sub>1</sub>-focal adhesion kinase pathway

黄文; 赵建武; 余佩琪; 任国胜

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Cyclic strain induces dermal fibroblasts orientation through integrin β₁-focal adhesion kinase pathway

更新时间：2026-01-09

- Cyclic strain induces dermal fibroblasts orientation through integrin β₁-focal adhesion kinase pathway
- Journal of Army Medical University Vol. 31, Issue 18, Pages: 1749-1752(2009)
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  CLC： R322.99;R329.26;R363.11
- Published：2009
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黄文,赵建武,余佩琪,任国胜.周期性张应变激活整合素β1-FAK信号途径调控皮肤成纤维细胞运动[J].陆军军医大学学报(原第三军医大学学报),2009,31(18):1749-1752.

　HUANG Wen,ZHAO Jian-wu,YU Pei-qi,REN Guo-sheng.Cyclic strain induces dermal fibroblasts orientation through integrin β1-focal adhesion kinase pathway[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(18):1749-1752.
黄文,赵建武,余佩琪,任国胜.周期性张应变激活整合素β1-FAK信号途径调控皮肤成纤维细胞运动[J].陆军军医大学学报(原第三军医大学学报),2009,31(18):1749-1752. DOI：

　HUANG Wen,ZHAO Jian-wu,YU Pei-qi,REN Guo-sheng.Cyclic strain induces dermal fibroblasts orientation through integrin β1-focal adhesion kinase pathway[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(18):1749-1752. DOI：

摘要

目的探讨周期性张应变对皮肤成纤维细胞的影响，寻找引起细胞运动的最佳张应变幅度及其信号转导机制。方法成纤维细胞培养于预敷胶原蛋白基质的6孔柔性培养板，对柔性培养孔施加10次/min的负压（-135 mmHg），每孔柔性板由中心到周边张应变幅度介于0%～24%。倒置显微镜观察细胞旋转角度，细胞免疫染色和激光共聚焦显微镜观察细胞整合素β

在细胞内的改变，免疫印迹观察细胞整合素β

表达和黏着斑激酶（FAK）磷酸化。结果发现15%的张应变幅度是诱导皮肤成纤维细胞运动的最低张应变幅度。机械张应变诱导成纤维细胞整合素β

在细胞再分布和FAK磷酸化，整合素抗体β

部分抑制了FAK磷酸化和细胞运动。结论机械张应变通过整合素β

-FAK途径引起皮肤成纤维细胞运动。

Abstract

Objective To assess the effects of cyclic stretch on fibroblast orientation in order to find the appropriate cyclic stretch to cause maximum fibroblast orientation and to explore the mechanism of cell signalling since cells are known to orient in response to the application of mechanical forces. Methods Human forehead dermal fibroblasts were seeded onto collagen coated flexible membranes. Membranes were then deformed at 10 cycles per minute by the application of 135 mmHg subatmospheric pressure. This corresponded to strain levels of 0% to 24% from the center to extremity of the flexible membrane. Cells orientation angles w

ere studied by inverted microscopy. Integrin β

distribution were studied with immunocytochemical staining and confocal microscopy. Integrin β

expression and focal adhesion kinase (FAK) phosphorylation were analyzed with Western blot analysis. Results A minimum of 15% cell stretch was required to significantly stimulate the fibroblast orientation response. Cyclic stretch induced integrin β

redistribution and FAK phosphorylation. Incubation of cells with anti-integrin β

prior to the application of stretch abrogated fibroblast orientation and FAK phosphory-lation. Conclusion Fibroblast orientation in response to cyclic stretch is mediated at least in part by integrin β

through phosphorylation of FAK.

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Grade Majoring in Clinical Medicine， School of Medicine， Anhui University of Science and Technology

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Cyclic strain induces dermal fibroblasts orientation through integrin β1-focal adhesion kinase pathway

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摘要

Abstract

关键词

Keywords

references

Cyclic strain induces dermal fibroblasts orientation through integrin β₁-focal adhesion kinase pathway