Objective    To investigate the effect of 1

25-(OH)

2

D

3

and salvia miltiorrhiza on T cell subsets and cytokines in rats after allogeneic bone marrow transplantation.     Methods    Female Wistar rats were used as recipients and male SD rats were used as donors. All Wista

r rats were divided into aGVHD group and intervention groups at random

and the intervention groups were further divided into 1

25-(OH)

2

D

3

group

salvia miltiorrhiza group and combination group. The changes of T cell subsets (CD4

+

and CD8

+

） and cytokine (IL-2

IFN-γ

IL-4 and IL-10) in every group were detected and measured.     Results    When the presentation of aGVHD was relatively conspicuous

CD4

+

and CD8

+

were increased

and the increase of CD4

+

was predominant. Compared with the difference prior to transplantation

the difference was statistically significant (P0.05). After being treated with the intervention program

the increase extent of CD4

+

and CD8

+

was lower than that of aGVHD group (P0.05)

in which the increase of CD4

+

was more conspicuous. Dynamic monitoring shows that the cytokine IL-2 and IFN-γ which were relevant to Th1 tended to go down in every intervention group

while the cytokine IL-10 relevant to Th2 tended to go up. The change was both relatively conspicuous on Day 21. Compared with the levels on Day 0

the difference was statistically significant (P0.05). But there was no obvious change for IL-4.     Conclusion    1

25-(OH)

2

D

3

and salvia miltiorrhiza can reduce the quantity of CD4

+

T lymphocytes

regulate the balance of Thl/Th2

restrict the proliferation and function exertion of Thl

promote the proliferation of Th2 cells and exert immune tolerance.