Objective   To construct the K562 cells that stably overexpresses Wnt5a protein

and to observe the effects of stable Wnt5a overexpression on the biological characters of K562 cells.    Methods   The plasmid vector pSEB-Wnt5a was constructed. K562 cells were infected by the retrovirus supernatant obtained through packaging

and the infected K562 cells were screened out. The expression of Wnt5a protein was examined with Western blot analysis. Cell counting

Wright’s staining and transmission electron microscopy (TEM) were used to observe the effects of Wnt5a overexpression on the biological characters of K562 cells.    Results   The plasmid pSEB-Wnt5a was constructed successfully

and the K562 cells infected by pSEB-Wnt5a retrovirus were screened out. Expression of Wnt5a protein in the K562 cells infected by pSEB-Wnt5a retrovirus was significantly higher than that in K562-vector cells. Compared with K562-vector cells

the proliferation of K562-Wnt5a cells was inhibited obviously

and the differentiation phenotype of K562-Wnt5a cells appeared.    Conclusion   The K562 cells that stably overexpresses Wnt5a protein are constructed successfully. Stable Wnt5a overexpression inhibits the proliferation of K562 cells and induces the differentiation of K562 cells

and it implies that Wnt5a is an antitumor factor in the pathogenesis of leukemia.