IL-8/CXCR<sub>2</sub> axis promotes proliferation, migration and VEGF expression of rabbit endothelial progenitor cells in vitro

毛梅; 吕学军; 王艺; 徐剑铖

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IL-8/CXCR₂ axis promotes proliferation, migration and VEGF expression of rabbit endothelial progenitor cells in vitro

更新时间：2026-01-09

- IL-8/CXCR₂ axis promotes proliferation, migration and VEGF expression of rabbit endothelial progenitor cells in vitro
- Journal of Army Medical University Vol. 31, Issue 24, Pages: 2429-2431(2009)
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  CLC： R322.12;R329.28;R392.12
- Published：2009
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毛梅,吕学军,王艺,徐剑铖.IL-8/CXCR2轴促进兔内皮祖细胞迁移、增殖及VEGF的表达[J].陆军军医大学学报(原第三军医大学学报),2009,31(24):2429-2431.

　MAO Mei,LU Xue jun,WANG Yi,XU Jian-cheng.IL-8/CXCR2 axis promotes proliferation, migration and VEGF expression of rabbit endothelial progenitor cells in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(24):2429-2431.
毛梅,吕学军,王艺,徐剑铖.IL-8/CXCR2轴促进兔内皮祖细胞迁移、增殖及VEGF的表达[J].陆军军医大学学报(原第三军医大学学报),2009,31(24):2429-2431. DOI：

　MAO Mei,LU Xue jun,WANG Yi,XU Jian-cheng.IL-8/CXCR2 axis promotes proliferation, migration and VEGF expression of rabbit endothelial progenitor cells in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(24):2429-2431. DOI：

摘要

目的观察不同浓度白介素-8（interleukin -8，IL-8）对体外培养兔外周血内皮祖细胞(endothelial progenitor cells

EPCs)生物学功能的影响，探讨趋化因子IL-8/CXCR

轴对EPCs血管生成功能的作用。方法密度梯度离心法获取兔外周血单个核细胞(mononuclear cells

MNCs)，接种至纤维连接蛋白(fibronectin

FN)包被的培养板上，培养7~10 d后收集贴壁细胞鉴定EPCs，并用不同浓度IL-8的培养基继续培养24 h后采用MTT比色法、改良的Boyden小室实验，观察EPCs的迁移和增殖能力；采用RT-PCR、Western blot方法检测CXCR

、VEGF mRNA及蛋白表达水平。结果 IL-8显著增加兔外周血EPCs的增殖和迁移能力（P0.05），且随着IL-8浓度升高而增强。IL-8促进CXCR

膜受体和VEGF的表达，且随着IL-8浓度增加而增加。结论 IL-8/CXCR

轴能显著促进EPCs的迁移、增殖及VEGF的表达。

Abstract

Objective To investigate the proliferation

migration and VEGF expression of rabbit peripheral blood endothelial progenitor cells (EPCs) after the treatment of interleukin-8 (IL-8) at different concentrations. Methods Total mononuclear cells (MNCs)were isolated from rabbit peripheral blood by density gradient centrifugation

and then the cells were plated on fibronectin-coated culture dishes. After cultivation for 7 d

the attached cells were incubated with IL-8 in a series of concent

rations for 24 h. The proliferation and migration activities of EPCs were measured with MTT assay and modified Boyden chamber assay

and reverse transcription (RT)-polymerase chain reaction (PCR) and Western blot analysis were used to detect the mRNA and protein expressions of CXCR2 and VEGF of EPCs. Results The proliferation

migration and CXCR2 and VEGF expressions of EPCs were regulated by IL-8

and enhanced with the increasing concentration of IL-8.  Conclusion The IL-8/CXCR

axis can significantly promote the EPCs proliferation

migration

and VGEF expression.

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IL-8/CXCR2 axis promotes proliferation, migration and VEGF expression of rabbit endothelial progenitor cells in vitro

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IL-8/CXCR₂ axis promotes proliferation, migration and VEGF expression of rabbit endothelial progenitor cells in vitro