Bacterial homology in sterile packages for war readiness under natural environment in inland region

张颖; 王仙园; 周娟

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Bacterial homology in sterile packages for war readiness under natural environment in inland region

更新时间：2026-01-09

- Bacterial homology in sterile packages for war readiness under natural environment in inland region
- Journal of Army Medical University Vol. 32, Issue 1, Pages: 16-18(2010)
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  CLC： R821.15;R117;R823
- Published：2010
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张颖,王仙园,周娟.内陆地区自然环境中战备消毒包内细菌同源性的实验研究[J].陆军军医大学学报(原第三军医大学学报),2010,32(01):16-18.

　Zhang Ying,Wang Xianyuan,Zhou Juan.Bacterial homology in sterile packages for war readiness under natural environment in inland region[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(01):16-18.
张颖,王仙园,周娟.内陆地区自然环境中战备消毒包内细菌同源性的实验研究[J].陆军军医大学学报(原第三军医大学学报),2010,32(01):16-18. DOI：

　Zhang Ying,Wang Xianyuan,Zhou Juan.Bacterial homology in sterile packages for war readiness under natural environment in inland region[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(01):16-18. DOI：

摘要

目的探讨内陆地区自然环境中战备消毒包内细菌来源。方法根据战备消毒包内蜡样芽孢杆菌、藤黄微球菌、科氏葡萄球菌解脲亚种筛选战备仓库空气同种细菌不同菌株，提取基因组DNA，以其为模板，对16S rRNA和23S rRNA 的转录间隔区（internal transcribed spacer

ITS）序列进行PCR扩增，将扩增产物回收测序，将测得的基因序列用Blast进行比对。结果蜡样芽孢杆菌、藤黄微球菌、科氏葡萄球菌解脲亚种DNA PCR扩增产物分别在850、1 200、1 000 bp 左右显现单一特异性条带；战备消毒包与空气同种细菌不同菌株之间的ITS序列相似性为98%～99%。结论内陆地区自然环境中战备消毒包内细菌来源于空气细菌。

Abstract

Objective To investigate the origin of bacteria in sterile packages for war readiness under natural environment in inland region. Methods Genomic DNAs from the Bacillus cereus

Micrococcus luteus and Staphylococcus cohnii ureal in sterile packages for war readiness and from air bacteria strains in war readiness warehouse were extracted

and then used as templates to amplify the 16S rRNA and 23S rRNA ITS (internal transcribed spacer) sequences by PCR. The PCR products were extracted and sequenced

and the sequences homology analysis was performed with BLAST. Results Amplification products of DNA PCR from Bacillus cereus

Micrococcus luteus and Staphylococcus cohnii ureal were about 800

1 200 and 1 000 bp respectively; The 16S rRNA-23S rRNA ITS sequences of bacterial strains in sterile packages shared 98% to 99% homology with those from the air bacteria strains in war readiness warehouse. Conclusion The bacteria in sterile packages for war readiness come from the air in war readiness warehouse under natural environment in inland region.

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