Objective    To identify the promoter sequence of endothelial-overexpressed lipopolysaccharide-associated factor 1 (EOLA1) gene and to elucidate the molecular mechanisms controlling EOLA1 expression.     Methods    A DNA fragment containing 1 723 bp 5′ upstream of the EOLA1 gene and the transcription start site was generated by polymerase chain reaction and then cloned into a luciferase reporter gene vector

pGL3-basic. The relative luciferase activities driven by this 5′-upstream fragment and a series of deletion mutants were measured in transiently transfected human ECV304 cells

respectively. At last

the 1 723 bp upstream of the EOLA1 gene was analyzed online with Cluster Buster.     Results    A fragment 785 bp upstream of the EOLA1 coding region was sufficient to promote transcription. Further deletion analysis of the 785 bp fragment indicated that a 68 bp element from -738 to -676 was important for EOLA1 transcription in ECV304 cells. The 1 723 bp sequence contains binding sites for Sp1 and Myf.     Conclusion    We map the EOLA1 promoter by deletion analysis and reveal that the proximal region (-738 to -676 bp)

which contains binding sites for Sp1 and Myf

is essential for human EOLA1 promoter activity in ECV304 cells.