Dexamethasone suppresses P2Y<sub>1</sub> receptor expression and TNF-α secretion of rat spinal dorsal horn astrocytes in vitro

施维维; 何文娟; 欧珊; 崔剑; 温慧中; 阮怀珍

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Dexamethasone suppresses P2Y₁ receptor expression and TNF-α secretion of rat spinal dorsal horn astrocytes in vitro

更新时间：2026-01-09

- Dexamethasone suppresses P2Y₁ receptor expression and TNF-α secretion of rat spinal dorsal horn astrocytes in vitro
- Journal of Army Medical University Vol. 32, Issue 1, Pages: 9-12(2010)
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  CLC： R338.15;R392.1;R977.11
- Published：2010
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施维维,何文娟,欧珊,崔剑,温慧中,阮怀珍.地塞米松抑制星形胶质细胞P2Y1受体表达及TNF-α分泌[J].陆军军医大学学报(原第三军医大学学报),2010,32(01):9-12.

　Shi Weiwei,He Wenjuan,Ou Shan,Cui Jian,Wen Huizhong,Ruan Huaizhen.Dexamethasone suppresses P2Y1 receptor expression and TNF-α secretion of rat spinal dorsal horn astrocytes in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(01):9-12.
施维维,何文娟,欧珊,崔剑,温慧中,阮怀珍.地塞米松抑制星形胶质细胞P2Y1受体表达及TNF-α分泌[J].陆军军医大学学报(原第三军医大学学报),2010,32(01):9-12. DOI：

　Shi Weiwei,He Wenjuan,Ou Shan,Cui Jian,Wen Huizhong,Ruan Huaizhen.Dexamethasone suppresses P2Y1 receptor expression and TNF-α secretion of rat spinal dorsal horn astrocytes in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(01):9-12. DOI：

摘要

目的观察不同浓度地塞米松对大鼠体外培养脊髓背角星形胶质细胞P2Y

受体表达及TNF-α分泌的影响。方法培养并纯化大鼠脊髓背角星形胶质细胞，地塞米松（终浓度分别为0.1、1、10 μmol/L）处理24 h后，采用免疫组织化学染色观察P2Y

受体的表达，双抗夹心间接酶联免疫吸附法测定上清液中TNF-α含量。结果地塞米松（0.1、1、10 μmol/L）作用24 h后，与对照组比较，脊髓背角星形胶质细胞P2Y

受体表达下调，并呈剂量依赖性（P0.01），TNF-α分泌量亦呈剂量依赖性下降（P0.01）。结论地塞米松能抑制体外培养大鼠脊髓背角星形胶质细胞P2Y

受体表达及TNF-α的分泌。

Abstract

Objective To investigate the influence of dexamethasone (DEX) on the changes of P2Y1 receptor (P2Y

R) and secretion of TNF-α in cultural astrocytes derived from suckling rat spinal dorsal horn. Methods Suckling SD rats at age of 3 d were anaesthetized and their spinal dorsal horn was resected and digested to obtain astrocytes of high purity with cell suspension culture. Astrocytes were classified into 4 groups

control

and treated with DEX at a final concentration of 0.1

1 and 10 μmol/L respectively. The expression of P2Y1R in the astrocytes and the concentration of TNF-α were detected by using immunohistochemical staining and ELISA respectively. R

esults The expression of P2Y

R was decreased with the increase of DEX final concentration compared with control group (P0.01)

and the contents of the TNF-α in the supernatant was para-llel to the trend of P2Y

R expression (P0.01). Conclusion DEX supresses the expression of P2Y

R and secretion of TNF-α in the cultured rat spinal dorsal horn astrocytes in a negatively dose-dependent manner.

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Dexamethasone suppresses P2Y1 receptor expression and TNF-α secretion of rat spinal dorsal horn astrocytes in vitro

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Dexamethasone suppresses P2Y₁ receptor expression and TNF-α secretion of rat spinal dorsal horn astrocytes in vitro