Objective    To investigate the effectiveness and mechanism of traditional Chinese ointment “CYG” on the promoting of wound healing of large-area full-thickness cutaneous defect.     Methods    Seven minipigs were employed in this study. Two full-thickness cutaneous wounds at a size of 10.5 cm×8.5 cm were produced on the dorsum area of each side close to the vertebral column in each animal. Among 28 wounds

14 right side wounds were treated with traditional Chinese ointment “CYG” as experimental group

and 14 left side wounds without treating as control. Subsequently the healing time

healing rate and healing pathological change of each wound surface during the 7 weeks’ healing procedure were observed

and immunohistochemistry staining was carried out with antibody against laminin to test the angiogenesis in the granulation tissue of the same wound surface. Immunohistochemistry staining and reverse transcriptase polymerase chain reaction (RT-PCR) were employed to examine the expression of transforming growth factor beta 1 (TGF-β1) of the same granulation tissue.     Results    In 7 to 44 d after injury

there were significant differences  in new area of wound and healing rate between 2 groups (P0.01). The healing time of experimental group was 32 to 34 d

and 10 to 12 d faster than that of control group. The wound surface of experimental groups grew with exquisite and planus textures and their granulation tissue

which contained a lot of laminin

fibroblasts

capillaries and collagen

and grew earlier than that of control groups. Expression of TGF-β1 at different interval time in experimental group was lower than that of control group

and this expression in wounds of the same group were ranked in the following order: intermediate stage (15 to 35 d postinjury)＞early phase(7 to 14 d postinjury)＞late stage (36 to 49 d postinjury) throughout healing procedure.     Conclusion    Traditional Chinese ointment CYG plays an effective role in promoting wound healing of large-area full-thickness cutaneous defect

and possibly suppresses the cicatrisation of this wound surface.