Norepinephrine improves  bone marrow mesenchymal stem cells proliferation through AR/PKC-dependent signaling

韩京; 冉新泽; 艾国平; 粟永萍

您当前的位置：

首页 >

文章列表页 >

Norepinephrine improves bone marrow mesenchymal stem cells proliferation through AR/PKC-dependent signaling

更新时间：2026-01-09

- Norepinephrine improves bone marrow mesenchymal stem cells proliferation through AR/PKC-dependent signaling
- Journal of Army Medical University Vol. 31, Issue 23, Pages: 2295-2298(2009)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R329.21;R329.28;R977.1
- Published：2009
- 稿件说明：
移动端阅览
韩京,冉新泽,艾国平,粟永萍.去甲肾上腺素促进骨髓间充质干细胞增殖的研究[J].陆军军医大学学报(原第三军医大学学报),2009,31(23):2295-2298.

　HAN Jing,RAN Xin-ze,AI Guo-ping,SU Yong-ping.Norepinephrine improves bone marrow mesenchymal stem cells proliferation through AR/PKC-dependent signaling[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(23):2295-2298.
韩京,冉新泽,艾国平,粟永萍.去甲肾上腺素促进骨髓间充质干细胞增殖的研究[J].陆军军医大学学报(原第三军医大学学报),2009,31(23):2295-2298. DOI：

　HAN Jing,RAN Xin-ze,AI Guo-ping,SU Yong-ping.Norepinephrine improves bone marrow mesenchymal stem cells proliferation through AR/PKC-dependent signaling[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(23):2295-2298. DOI：

摘要

目的观察去甲肾上腺素对骨髓间充质干细胞增殖的影响及其作用途径。方法将BMSCs细胞随机分为3组：①正常对照组；②去甲肾上腺素（10

-6

~10

-4

mol/L）处理组；③酚妥拉明（10

-6

mol/L）采+去甲肾上腺素（10

-5

mol/L）处理组。采用

H-TdR掺入实验检测不同实验组对BMSCs增殖的影响；免疫组化和免疫印记法检测去甲肾上腺素对蛋白激酶C的作用。结果 ①10

-6

~10

-4

mol/L的去甲肾上腺素作用8 h后均促进了骨髓间充质干细胞的增殖，并且在10

-5

mol/L 时去甲肾上腺素对骨髓间充质干细胞的促增殖作用最为显著；②酚妥拉明阻断了去甲肾上腺素对骨髓间充质干细胞的促增殖作用；③去甲肾上腺素诱导蛋白激酶C从细胞质到细胞膜转位，酚妥拉明抑制了去甲肾上腺素诱导的蛋白激酶C激活。结论去甲肾上腺素能够促进骨髓间充质干细胞的增殖，并且这种促增殖作用是通过肾上腺素能受体、蛋白激酶C信号通路来调节的。

Abstract

Objective To investigate the effects of norepinephrine on the proliferation of bone marrow mesenchymal stem cells (BMSCs) and the related signaling molecules involved in BMSCs. Methods

H-thymidine incorporation assay was performed to investigate the effect of norepinephrine on BMSCs proliferation

in the presence of various concentrations (10

-6

to 10

-4

mol/L) for 8 h or with phentolamine (10

-6

mol/L) for 30 min. Effect of norepinephrine on PKC activation was detected by immunofluorescent stai

ning and Western blot analysis. Results Norepinephrine treatment (10

-6

to10

-4

mol/L) for 8 h increased the

H-thymidine incorporation in a dose-dependent manner. The maximal stimulatory effect was observed at 10

-5

mol/L of norepinephrine. The stimulatory effect of norepinephrine on cell proliferation was blocked by co-incubation with phentolamine. Norepinephrine induced PKC translocation to the cell membrane

which was blocked by pretreatment with phentolamine for 30 min. Conclusion This stimulatory effect of norepinephrine on cell proliferation is mediated through AR/PKC-dependent signaling.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Role of microfilament depolymerization in osteogenic differentiation of bone marrow mesenchymal stem cells promoted by low intensity pulsed ultrasound

Bone marrow mesenchymal stem cell-derived exosomes promote proliferation, invasion and migration of renal cancer cells by regulating cholestanol metabolism

Effect of BMSCs-derived exosomes on expression of lncRNAs in rat carotid artery after balloon injury

Mechanism of exosomes released from bone marrow stem cells regulating proliferation of cardiac microvascular endothelial cells

Effect of bone marrow mesenchymal stem cells modified with Toll-like receptor 4 on cardiac function in rats after acute myocardial infarction

Related Author

杨丽

姚欢

朱慧

何依蔓

杨珂

王冬

王和西

刘学洋

Related Institution

No data

⁰