Management of corneal alkali burn with transplantation of limbal epithelial cells and decellularized porcine cornea in a rabbit model

郭梁; 胡丹

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Management of corneal alkali burn with transplantation of limbal epithelial cells and decellularized porcine cornea in a rabbit model

更新时间：2026-01-09

- Management of corneal alkali burn with transplantation of limbal epithelial cells and decellularized porcine cornea in a rabbit model
- Journal of Army Medical University Vol. 31, Issue 10, Pages: 959-962(2009)
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  CLC： R318.08;R772.2;R779.65
- Published：2009
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郭梁,胡丹.角膜缘上皮细胞与脱细胞角膜材料移植修复碱烧伤角膜的实验研究[J].陆军军医大学学报(原第三军医大学学报),2009,31(10):959-962.

　GUO Liang,HU Dan.Management of corneal alkali burn with transplantation of limbal epithelial cells and decellularized porcine cornea in a rabbit model[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(10):959-962.
郭梁,胡丹.角膜缘上皮细胞与脱细胞角膜材料移植修复碱烧伤角膜的实验研究[J].陆军军医大学学报(原第三军医大学学报),2009,31(10):959-962. DOI：

　GUO Liang,HU Dan.Management of corneal alkali burn with transplantation of limbal epithelial cells and decellularized porcine cornea in a rabbit model[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(10):959-962. DOI：

摘要

目的探讨应用角膜缘上皮细胞(limbal epithelial cells，LECs)及脱细胞角膜材料(decellularized porcine cornea

DPC) 移植修复碱烧伤兔角膜的疗效。方法培养同种异体兔LECs，鉴定其中的角膜缘干细胞(limbal stem cells，LSCs)，制备DPC作为支架材料，将LECs和DPC两者复合培养后板层移植到碱烧伤的兔眼角膜，观察移植后4个月内的角膜修复情况。结果免疫细胞化学及RT-PCR检测培养的LECs中有表达ABCG2的LSCs。大体观察LECs-DPC移植后碱烧伤角膜的混浊度减轻，新生血管消退。HE、Masson Trichrome染色显示移植后上皮面的细胞复层化生长，炎性细胞浸润减少，DPC纤维整合于碱烧伤的角膜植床，角膜胶原排列趋于规则。透射电镜检查有桥粒及微绒毛结构形成。结论体外培养的同种异体角膜缘上皮细胞联合脱细胞角膜材料移植能够修复碱烧伤的兔眼角膜。

Abstract

Objective To investigate the therapeutic effect of transplantation of limbal epithelial cells and decellularized porcine cornea construct in rabbits with corneal alkali burn. Methods Allogeneic rabbit limbal epithelial cells were cultured and identified. Porcine cornea scaffold was fabricated by decellularization.The LECs-DPC construct was cultured and transplanted to the alkali-burned corneal surface of rabbits (4month follow-up). Results Immunocytochemistry and RT-PCR analyses revealed the presence of ABCG2 positive limbal stem cells. A general view showed the corneas were clear and vessel-free postoperatively . HE and Masson Trichrome staining results indicated the epithelial stratification

fewer cellular infiltration

DPC’ integration into host corneal tissues

and more regular collagen configuration. Transmission electron microscopy exhibited formation of desmosomes and microvilli. Conclusion Allogeneic limbal epithelial cells transplantation using DPC as a carrier can restore corneal clarity after alkali burn in a rabbit model.

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