Effect of cocultured  pulmonary microvascular endothelial cells on cytokine expressions in pulmonary arterial smooth muscle cells after hypoxic exposure

张志远; 钱频; 王建春; 程序; 刘婷; 钱桂生; 王关嵩

您当前的位置：

首页 >

文章列表页 >

Effect of cocultured pulmonary microvascular endothelial cells on cytokine expressions in pulmonary arterial smooth muscle cells after hypoxic exposure

更新时间：2026-01-09

- Effect of cocultured pulmonary microvascular endothelial cells on cytokine expressions in pulmonary arterial smooth muscle cells after hypoxic exposure
- Journal of Army Medical University Vol. 31, Issue 13, Pages: 1302-1304(2009)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R322.121;R364.4;R563.02
- Published：2009
- 稿件说明：
移动端阅览
张志远,钱频,王建春,程序,刘婷,钱桂生,王关嵩.复合培养对低氧时肺动脉平滑肌细胞表达细胞因子的调节[J].陆军军医大学学报(原第三军医大学学报),2009,31(13):1302-1304.

　ZHANG Zhi-yuan,QIAN Pin,WANG Jian-chun,CHENG Xu,LIU Ting,QIAN Gui-sheng,WANG Guan-song.Effect of cocultured pulmonary microvascular endothelial cells on cytokine expressions in pulmonary arterial smooth muscle cells after hypoxic exposure[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(13):1302-1304.
张志远,钱频,王建春,程序,刘婷,钱桂生,王关嵩.复合培养对低氧时肺动脉平滑肌细胞表达细胞因子的调节[J].陆军军医大学学报(原第三军医大学学报),2009,31(13):1302-1304. DOI：

　ZHANG Zhi-yuan,QIAN Pin,WANG Jian-chun,CHENG Xu,LIU Ting,QIAN Gui-sheng,WANG Guan-song.Effect of cocultured pulmonary microvascular endothelial cells on cytokine expressions in pulmonary arterial smooth muscle cells after hypoxic exposure[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(13):1302-1304. DOI：

摘要

目的研究低氧对肺动脉平滑肌细胞（pulmonary arterial smooth muscle cells，PASMCs）白细胞介素-1β、4、10、13（IL-1β、4、10、13）活性的影响，及复合培养下肺微血管内皮细胞（pulmonary microvascular endothelial cells，PMVECs）对其调控作用。方法大鼠PASMCs单独培养或与PMVECs复合培养，分为正常组、低氧作用2 h（H

）、6h（H

）、12 h（H

）、24 h(H

)组，采用ELISA法测定各组培养细胞上清中IL-1β、IL-4、IL-10、IL-13的活性。结果低氧刺激后PASMCs的IL-1β、IL-4、IL-10、IL-13活性2 h开始升高，6 h达高峰，12 h降低；各时相点复合培养组均低于对应的单独培养组（P0.01）。 结论 低氧可以激活PASMCs的IL-1β、IL-4、IL-10、IL-13活性，在复合培养条件下，PMVECs对此效应具有下调性影响。

Abstract

Objective To study the effect of hypoxia on the expressions of interleukin-1β

and 13 (IL-1β

IL-4

IL-10 and IL-13) in pulmonary arterial smooth muscle cells (PASMCs) and the regulative role of cocultured pulmonary microvascular endothelial cells (PMVECs). Methods Rat PASMCs were cocultured with or without rat PMVECs

and randomly divided into 5 groups

normal group (N)

2-hour hypoxia (H2)

6-hour hypoxia (H6)

12-hour hypoxia (H12)

and 24-hour hypoxia (H24). The expressions of IL-1β

IL-4

IL-10 and IL-13 in the supernatant were detected with ELISA. Results The expression levels of IL-1β

IL-4

IL-10 and IL-13 was increased in H2 group

peaked in H6 group

and then decreased in H12 group. However

the expression levels of above-mentioned cytokines in cocultured groups were significantly lower than those in corresponding hypoxia group. Conclusion Hypoxia can enhance activities of IL-1β

IL-4

IL-10

and IL-13 in PASMCs

but this enhancement can be down-regulated by cocultured PMVECs.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Chronic hypoxia reduces cold tolerance via hepatic ANGPTL3-driven mitochondrial damage and adipose thermogenesis suppression

Mdivi-1 improves cognitive dysfunction in immature SD rats induced by hypoxia combined with propofol by inhibiting excessive mitophagy

Molecular mechanism of hypoxia-induced spermatogenesis impairment by inhibiting the stability of sperm cilial microtubules

Plateau hypoxia improves tumor immune microenvironment and inhibits subcutaneous tumor growth of colorectal cancer

Hypoxia aggravates propofol-induced hippocampal neuron injury in immature SD rats through ferroptosis

Related Author

HUANG Yujie

HUANG Lingfang

ZHEGN Minghai

XIONG Kun

TAN Xiaoling

XU Yao

YANG Shun

YANG Fei

Related Institution

Department of Frigid Zone Medicine， College of High Altitude Military Medicine, School of Nursing， Army Medical University （Third Military Medical University）

Key Laboratory of Extreme Environmental Medicine of Ministry of Education, School of Nursing， Army Medical University （Third Military Medical University）

Department of Clinical Nursing, School of Nursing， Army Medical University （Third Military Medical University）

Department of Anesthesiology Children’s Hospital of Chongqing Medical University， National Clinical Research Center for Child Health and Disorders， Ministry of Education Key Laboratory of Child Development and Disorders， Chongqing Key Laboratory of Child Neurodevelopment and Cognitive Disorders

Department of Pathophysiology， Key Laboratory of High Altitude Medicine， Key Laboratory of Extreme Environmental Medicine of Ministry of Education， Faculty of High Altitude Military Medicine， Army Medical University （Third Military Medical University）

⁰