HPV16E6 transfection improves growth and cell division of cervical carcinoma cell line C33A

杨秀坤; 陈宣男; 姜铮

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HPV16E6 transfection improves growth and cell division of cervical carcinoma cell line C33A

更新时间：2026-01-09

- HPV16E6 transfection improves growth and cell division of cervical carcinoma cell line C33A
- Journal of Army Medical University Vol. 31, Issue 9, Pages: 835-838(2009)
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  CLC： R394.2;R730.23;R737.33
- Published：2009
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杨秀坤,陈宣男,姜铮.pcDNA3转染HPV16E6基因对人宫颈癌细胞C33A细胞生长及周期的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(09):835-838.

　YANG Xiu-kun,CHEN Xuan-nan,JIANG Zheng.HPV16E6 transfection improves growth and cell division of cervical carcinoma cell line C33A[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(09):835-838.
杨秀坤,陈宣男,姜铮.pcDNA3转染HPV16E6基因对人宫颈癌细胞C33A细胞生长及周期的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(09):835-838. DOI：

　YANG Xiu-kun,CHEN Xuan-nan,JIANG Zheng.HPV16E6 transfection improves growth and cell division of cervical carcinoma cell line C33A[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(09):835-838. DOI：

摘要

目的探讨HPV16E6对宫颈癌C33A细胞生长增殖的影响。方法通过脂质体法将HPV16E6基因稳定转染宫颈癌C33A细胞，RT-PCR及Western blot分别检测转染细胞中HPV16E6 mRNA和蛋白表达水平，流式细胞仪检测HPV16E6基因转染对C33A细胞生长增殖与细胞周期的影响。结果稳定转染获得了稳定表达pcDNA3-HPV16E6质粒的C33A细胞克隆(C33A-E6细胞，即pcDNA3/HPV16E6/C33A组)及稳定复制空载体质粒的细胞克隆(C33A-P细胞，即pcDNA3/C33A组)，并将未转染的C33A细胞作为对照组。pcDNA3/HPV16E6/C33A组细胞的生长速度比pcDNA3/C33A组和对照组明显加快(P0.05)。在转染了HPV16E6基因的C33A细胞中，其DNA合成前期G

和静止期G

期)细胞百分率(39.3%)明显低于pcDNA3/C33A组(53.7%)和对照组(55.4%)(P0.01);而处于S期细胞的百分率(38.3%)、DNA合成后期G

及分裂期M的细胞百分率(G

/M：32.9%)，均明显高于pcDNA3/C33A组(S:29.4%;G

/M:17.0%)和对照组(S:28.0%;G

/M:16.6%)(P0.01)。pcDNA3/C33A组和对照组细胞周期的各期分布无明显差异(P0.05)。结论 HPV16E6可能通过某种途径参与了C33A细胞的周期调控，促进细胞分裂，从而促进C33A细胞的增殖。

Abstract

Objective To investigate the transfection of human papilloma virus types 16E6 gene on the proliferation and apoptosis of cervical carcinoma cell line C33A. Methods Plasmid pcDNA3-HPV16E6 was stably transfected into C33A cells by liposome

and the expression of HPV16E6 mRNA and protein in the transfected ce

lls

the cells transfected with blank vector and the cells without transfection were identified by RT-PCR and Western blot analysis. The growth

proliferation and cell cycles of the 3 kinds of C33A cells were by cell growth curve plotting and flow cytometry analysis. Results C33A cells were transfected stably by liposome with plasmid pcDNA3-HPV16E6 (C33A-E6 cells)and plasmid pcDNA3 (C33A-P cells). The growth of C33A-E6 cells was more rapid than those of C33A-P and C33A cells (P0.05)

indicating that HPV16E6 promoted the growth and proliferation of C33A cells. In C33A-E6 cells

the percentage of the cells at G

phase (39.3%) were significantly lower than those of C33A-P cells (53.7%) and C33A cells (55.4%) (P0.01). But the percentage of the cells at S phase (38.3%) and at G

/M phase (32.9%) were distinctly higher than those of C33A-P cells (29.4% and 17.0%) and C33A cells (28.0% and 16.6%) (P0.0l). However

there was significantly difference between C33A-P cells and C33A cells (P0.05). Conclusion HPV16E6 is involved in the cell cycle probably through some signal pathways

and promotes cell division and proliferation of C33A cells.

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