Expression and significance of peripheral CXCL9 and CXCR3 in rats with acute renal allografts rejection

李家兵; 何云锋; 夏康; 吴小侯

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Expression and significance of peripheral CXCL9 and CXCR3 in rats with acute renal allografts rejection

更新时间：2026-01-09

- Expression and significance of peripheral CXCL9 and CXCR3 in rats with acute renal allografts rejection
- Journal of Army Medical University Vol. 31, Issue 9, Pages: 828-830(2009)
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  CLC： R392.4;R446.119;R692
- Published：2009
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李家兵,何云锋,夏康,吴小侯.外周血CXCL9和CXCR3在大鼠肾移植急性排斥反应的表达及意义[J].陆军军医大学学报(原第三军医大学学报),2009,31(09):828-830.

　LI Jia-bing,HE Yun-feng,XIA Kang,WU Xiao-hou.Expression and significance of peripheral CXCL9 and CXCR3 in rats with acute renal allografts rejection[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(09):828-830.
李家兵,何云锋,夏康,吴小侯.外周血CXCL9和CXCR3在大鼠肾移植急性排斥反应的表达及意义[J].陆军军医大学学报(原第三军医大学学报),2009,31(09):828-830. DOI：

　LI Jia-bing,HE Yun-feng,XIA Kang,WU Xiao-hou.Expression and significance of peripheral CXCL9 and CXCR3 in rats with acute renal allografts rejection[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(09):828-830. DOI：

摘要

目的探讨大鼠肾移植手术前后趋化因子CXCL9及其受体CXCR3 mRNA的动态变化在术后急性排斥早期诊断的作用。方法以BN和LEW大鼠作为供体，LEW大鼠作为受体，建立大鼠同种异体肾移植模型。采用ELISA法和逆转录聚合酶链反应（RT-PCR）分别动态检测大鼠术前后血清CXCL9和外周血淋巴细胞CXCR3 mRNA的变化。同时检测血清肌酐水平。结果实验组大鼠血清CXCL9、外周血淋巴细胞CXCR3 mRNA及血清肌酐表达均明显升高，与对照组比较差异有统计学意义（P0.01）。术前实验组与对照组血清CXCL9和外周血淋巴细胞CXCR3 mRNA表达比较无统计学差异（P＞0.05），术后第1、3、5、7天实验组均高于对照组，差异有统计学意义（P0.05）。术前及术后第1天实验组和对照组血清肌酐表达比较无统计学差异

术后第3、5、7天实验组均高于对照组，差异有统计学意义（P0.05）。结论血清趋化因子CXCL9和外周血淋巴细胞CXCR3 mRNA的表达与排斥反应密切相关，可以作为早期诊断急性排斥反应的辅助敏感指标。

Abstract

Objective To evaluate dynamic changes of chemokine CXCL9 and chemokine receptor CXCR3 to predict the early acute rejection in rats after renal transplantation. Methods Renal allogeneic grafting was performed on Brown Norway or Lewis rats as donors and Lewis rats as recipients. CXCL9 in serum was measured by ELISA and CXCR3 mRNA in peripheral blood lymphocytes by RT-PCR at the time points of just before and 1

and 7 d after operation. Serum creatinine (Cr) was measured simultaneously. Results The serum contents of CXCL9 and Cr

and CXCR3 mRNA level in allograft group were significantly higher than those in isograft group (P0.01). There was no significant difference on CXCL9 content and CXCR3 mRNA level in both allograft and isograft groups before operation

and the difference become significantly higher on the day 1

5 and 7 after operation in allograft group than that in isograft group (P0.05). No difference was seen on the serum Cr level in allograft and isograft groups before and 1 d after operation

but the difference was observed on 3

5 and 7 d after operation between the 2 groups (P0.05). Conclusion Serum CXCL9 content and CXCR3 mRNA level in peripheral blood lymphocytes are sensitive predictors for the early diagnosis of acute rejection.

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