Effects of survivin gene silencing on growth and apoptosis of adenoid cystic carcinoma cell line

汪欣; 杨军; 许波; 熊宇; 张从纪

您当前的位置：

首页 >

文章列表页 >

Effects of survivin gene silencing on growth and apoptosis of adenoid cystic carcinoma cell line

更新时间：2026-01-09

- Effects of survivin gene silencing on growth and apoptosis of adenoid cystic carcinoma cell line
- Journal of Army Medical University Vol. 31, Issue 11, Pages: 1053-1056(2009)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R394.2;R73-362;R730.23
- Published：2009
- 稿件说明：
移动端阅览
汪欣,杨军,许波,熊宇,张从纪.survivin基因沉默对腺样囊性癌细胞生长和凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(11):1053-1056.

　WANG Xin,YANG Jun,XU Bo,XIONG Yu,ZHANG Cong-ji.Effects of survivin gene silencing on growth and apoptosis of adenoid cystic carcinoma cell line[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(11):1053-1056.
汪欣,杨军,许波,熊宇,张从纪.survivin基因沉默对腺样囊性癌细胞生长和凋亡的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(11):1053-1056. DOI：

　WANG Xin,YANG Jun,XU Bo,XIONG Yu,ZHANG Cong-ji.Effects of survivin gene silencing on growth and apoptosis of adenoid cystic carcinoma cell line[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(11):1053-1056. DOI：

摘要

目的探讨RNA干扰下调survivin基因表达后腺样囊性癌细胞生物学行为变化及其与caspase-3基因表达的相关性。方法设计、合成小干扰RNA（small interfering RNA，siRNA），构建表达载体pGenesil-shRNA-survivin，将重组质粒导入人腺样囊性癌ACC-2细胞株

G418筛选阳性克隆。采用逆转录-聚合酶链反应(RT-PCR)、Western blot分别检测转染后的ACC-2细胞中survivin、caspase-3 mRNA及蛋白表达的变化，用噻唑蓝(MTT)法检测细胞的增殖，原位细胞凋亡(TUNEL)法及透射电子显微镜研究细胞凋亡。结果测序证实表达载体构建成功，在pGenesil-shRNA-survivin的ACC-2细胞株中

survivin mRNA及蛋白表达明显降低(P0.05)

caspase-3 mRNA及蛋白表达明显增加(P0.05)，survivin与caspase-3的mRNA和蛋白表达呈显著负相关（r=-0.333

P0.05）。ACC-2细胞增殖受到抑制，透射电镜及TUNEL法可见细胞出现凋亡。结论成功构建了survivin基因的RNAi真核表达载体，pGenesil-shRNA-survivin重组质粒能有效抑制腺样囊性癌细胞生长。

Abstract

Objective To investigate the effects of survivin down-regulation on the biological behavior of adenoid cystic carcinoma cells and the relationship with caspase-3. Methods Small interfering RNA (siRNA) was designed and synthesized for the construction of eukaryotic expression vector pGenesil-shRNA-survivin. The recombinant plasmid was transfected into ACC-2 cells. The positive clones were screened with G418. The expressions of survivin and caspase-3 mRNA and protein were detected by RT-PCR and Western blot. The proliferation of adenoid cystic carcinoma cells was detected by MTT. Cell apoptosis was detected by TUNEL assay and transmission electron microscopy. Results The recombinant plasmid was successfully constructed. Down-regulation was found in the expressions of survivin mRNA and protein (P0.05)

but up-regulation in the expressions of caspase-3 mRNA and protein (P0.05). There was a negative correlation between the mRNA and protein expression of caspase-3 and survivin (r=-0.333

P0.05). ACC-2 cell proliferation was inhibited. TUNEL assay and transmission electron microscopy showed typical apoptosis of cells. Conclusion The RNAi eukaryotic vector pGenesil-shRNA-survivin was constructed successfully. Recombinant plasmid pGenesil-shRNA-survivin can inhibit the proliferation of adenoid cystic carcinoma cells.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Inhibitory effect of survivin-targeting siRNA on transplanted tumor growth of renal carcinoma in nude mice

RNAinterfered survivin gene increases sensitivity of SKBr-3 cells to doxorubicin: an in vitro study

Effect of RNA-silenced survivin gene on sensitivity of K562 cells to adriamycin

Effect of enhanced survivin expression on lymphocyte proliferation and function in K562 cells

Effects of RNAi-silenced survivin gene on bladder cancer cell lines EJ

Related Author

刘富

胡自力

李庆霞

王娟

赵文清

刘家云

黄红艳

张明

Related Institution

No data

⁰