Down-regulation of CXCR4 expression inhibits invasion and VEGF production of glioma stem cells

赵林涛; 卞修武; 姜建勇; 王斌; 平轶芳; 余时沧

您当前的位置：

首页 >

文章列表页 >

Down-regulation of CXCR4 expression inhibits invasion and VEGF production of glioma stem cells

更新时间：2026-01-09

- Down-regulation of CXCR4 expression inhibits invasion and VEGF production of glioma stem cells
- Journal of Army Medical University Vol. 31, Issue 11, Pages: 1049-1052(2009)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R394-33;R73-34;R730.264
- Published：2009
- 稿件说明：
移动端阅览
赵林涛,卞修武,姜建勇,王斌,平轶芳,余时沧.下调CXCR4表达对胶质瘤干细胞侵袭能力和VEGF分泌量的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(11):1049-1052.

　ZHAO Lin-tao,BIAN Xiu-wu,JIANG Jian-yong,WANG Bin,PING Yi-fang,YU Shi-cang.Down-regulation of CXCR4 expression inhibits invasion and VEGF production of glioma stem cells[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(11):1049-1052.
赵林涛,卞修武,姜建勇,王斌,平轶芳,余时沧.下调CXCR4表达对胶质瘤干细胞侵袭能力和VEGF分泌量的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(11):1049-1052. DOI：

　ZHAO Lin-tao,BIAN Xiu-wu,JIANG Jian-yong,WANG Bin,PING Yi-fang,YU Shi-cang.Down-regulation of CXCR4 expression inhibits invasion and VEGF production of glioma stem cells[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(11):1049-1052. DOI：

摘要

目的观察下调胶质瘤干细胞CXCR4表达后对其侵袭能力和血管内皮生长因子（vascular endothelial growth factor，VEGF）分泌量的影响。方法采用间接免疫荧光标记观测U87细胞及其颅内移植瘤标本中胶质瘤干细胞标记物CD133和CXCR4共表达情况；分别从稳定转染CXCR4小干扰RNA质粒的U87细胞和稳定转染阴性对照质粒的U87细胞中培养出胶质瘤干细胞球，并对其进行鉴定，观察两者CXCR4的表达情况；通过Transwell小室比较两者的侵袭能力；采用酶联免疫吸附实验（ELISA）检测两者细胞培养上清内VEGF的含量。结果 U87细胞及其移植瘤中均可检测到CXCR4与CD133共表达，下调胶质瘤干细胞CXCR4表达后，胶质瘤干细胞的体外侵袭能力明显下降[干扰组：（17.0±5.8），对照组：（71.5±8.7）

P0.05]，VEGF分泌明显减少[干扰组：24 h （690±24）pg/ml，48 h （1 504±53）pg/ml；对照组：24 h （850±25）pg/ml，48 h （2 001±150）pg/ml

P0.05]。结论胶质瘤干细胞表达CXCR4，下调其表达可抑制胶质瘤干细胞的侵袭和促血管生成能力，提示CXCR4可能成为针对胶质瘤干细胞进行治疗的靶点。

Abstract

Objective To observe the effects of CXCR4 downregulation on the invasion capacity and VEGF secretion of glioma stem cells (GSCs). Methods Coexpression of CXCR4 and CD133 in U87 cells and their xenografts grown in nude mice brain was detected by indirect immunofluorescence

under a laser scanning microscope. Tumorspheres were isolated from control U87 cells and CXCR4-siRNA U87 cells

respectively. We investigated the expression of CD133

nestin and CXCR4 in tumorspheres by laser scanning microscopy. Effects of CXCR4 downregulation on invasion and VEGF production of GSCs were detected by transwell assay and ELISA respectively. Results CXCR4 and CD133 were coexpressed in U87 cells and their xenografts. Downregulation of CXCR4 expression inhibits the invasion capacity [siRNA:（17.0±5.8）

control:（71.5±8.7）

P0.05] and VEGF secretion of GSCs[siRNA: 24 h （690±24）pg/ml，48 h （1 504±53）pg/ml

control: 24 h （850±25）pg/ml，48 h （2 001±150）pg/ml

P0.05]. Conclusion GSCs express CXCR4

and downregulation of CXCR4 expression attenuates the invasion and pro-angiogenic capacity of GSCs

which suggests that CXCR4 expressed on GSCs may be a potential target of glioma therapy.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Ginsenoside-Rg1 promotes angiogenesis in rats with acute myocardial ischemia

Ecdysterone induces angiogenesis and protects neurologic function after focal cerebral ischemia in rats

Effects of HYAL1 gene overexpression on invasive, angiogenic and proliferative ability of breast cancer cells

Role of CXCR4 in lymph node metastasis promoted by VEGF-C in gastric carcinoma

Gene silencing of VEGF inhibits growth and angiogenesis of human colon cancer HCT116 implanted in nude mice

Related Author

张庆勇

陈燕萍

刘芬

陈霞

罗春霞

陈康宁

冯华

鲁开智

Related Institution

Department of Gastroenterology, Qingdao Central Hospital of Rehabilitation University

Endoscopy Center, Qingdao Central Hospital of Rehabilitation University

Department of Pathology， Second Affiliated Hospital， Army Medical University （Third Military Medical University）

School of Medicine， Shihezi University

Department of Stomatology， the First Affiliated Hospital of Shihezi University

⁰