Effects of uric acid on TGF-β1 and Smad3 expression in human urethral fibroblasts

郭旭; 卢根生

您当前的位置：

首页 >

文章列表页 >

Effects of uric acid on TGF-β1 and Smad3 expression in human urethral fibroblasts

更新时间：2026-01-09

- Effects of uric acid on TGF-β1 and Smad3 expression in human urethral fibroblasts
- Journal of Army Medical University Vol. 31, Issue 11, Pages: 1045-1048(2009)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R363.13;R619.6;R693.5
- Published：2009
- 稿件说明：
移动端阅览
郭旭,卢根生.尿酸对人尿道成纤维细胞TGF-β1、Smad3的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(11):1045-1048.

　GUO Xu,LU Gen-sheng.Effects of uric acid on TGF-β1 and Smad3 expression in human urethral fibroblasts[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(11):1045-1048.
郭旭,卢根生.尿酸对人尿道成纤维细胞TGF-β1、Smad3的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(11):1045-1048. DOI：

　GUO Xu,LU Gen-sheng.Effects of uric acid on TGF-β1 and Smad3 expression in human urethral fibroblasts[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(11):1045-1048. DOI：

摘要

目的观察人尿道成纤维细胞在尿液、尿酸刺激下TGF-β1、Smad3的表达变化，探讨尿道瘢痕的形成机制。方法体外培养人尿道成纤维细胞，以尿液、350 μmol/L浓度的尿酸刺激溶液刺激人尿道成纤维细胞，通过免疫荧光染色和逆转录-聚合酶链反应（RT-PCR）分别检测各组细胞TGF-β1、Smad3因子表达变化

ELISA法比较各组细胞上清液Ⅰ型胶原蛋白差异。结果免疫荧光检测提示TGF-β1与Smad3蛋白在人尿道成纤维细胞中均有表达，尿液及生理浓度的尿酸刺激后，TGF-β1与Smad3的mRNA表达上调，蛋白表达明显升高（P0.05）；ELISA法检测显示，尿液和尿酸刺激人尿道成纤维细胞24、48 h后，细胞上清液中Ⅰ型胶原蛋白含量显著升高(P0.05)。结论尿酸刺激引起人尿道成纤维细胞TGF-β1、Smad3的表达升高；尿酸可能对尿道瘢痕的形成起促进作用。

Abstract

Objective To observe the effects of urine on TGF-β1 and Smad3 expression in cultured human urethral fibroblasts and explore the mechanism of urethral scar fibrosis. Methods The human urethral fibroblasts were cultured and stimulated by urine or 350 μmol/L uric acid. The mRNA and protein expression of TGF-β1 and Smad3 were measured by PT-PCR and immunofluorescent staining

respectively. Collagen Ⅰlevels in the supernatants in different groups were detected by ELISA. Results TGF-β1 and Smad3 were detected in human urethral fibroblasts by immunofluorescent staining. After being presented with urine or uric acid at physiological concentration

both TGF-β1 and Smad3 in human urethral fibroblasts were increased significantly at both the protein and mRNA levels（P0.05）. By ELISA

the upregulation of Collagen Ⅰ in the supernatant was also discovered at 24 or 48 h after stimulation with urine or uric acid. Conclusion Uric acid stimulation could increase the expression of TGF-β1 and Smad3. Uric acid probably take part in promotion of urethral scar fibrosis.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Change in serum uric acid level and its influencing factors among military personnel during long-term maritime mission

Functional brain abnormalities in male patients with hyperuricemia: a study by resting-state functional magnetic resonance imaging

Effects of hypoxia on expression of formyl peptide receptor 1 in rat lung fibroblasts in vitro

Phenotypes of acute renal function injuries in different acute high-altitude diseases

High uric acid induces epithelial-mesenchymal transition of renal tubular epithelial cells via TLR4/NF-κB signaling pathway

Related Author

SHI Lifeng

WU Yan

WANG Guangyong

ZHANG Shaoyu

WANG Fang

GUO Tao

TANG Guangman

LI Lan

Related Institution

Outpatient Department for Military Perfonnel， No. Hospital of Joint Logistic Support Force

Department of Gastroenterology， Naval Medical Center of PLA

Hospital of Troop

Department of Orthopedics， No. Hospital of Joint Logistic Support Force

⁰