Behavioral and pathologic changes of chronic cerebral hypoperfusion in mice

段炜; 郑健; 桂莉; 田洪

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Behavioral and pathologic changes of chronic cerebral hypoperfusion in mice

更新时间：2026-01-09

- Behavioral and pathologic changes of chronic cerebral hypoperfusion in mice
- Journal of Army Medical University Vol. 31, Issue 9, Pages: 813-817(2009)
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  CLC： R-332;R363;R743.02
- Published：2009
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段炜,郑健,桂莉,田洪.小鼠慢性脑血流低灌注状态的行为学检测及病理学观察[J].陆军军医大学学报(原第三军医大学学报),2009,31(09):813-817.

　DUAN Wei,ZHENG Jian,GUI Li,TIAN Hong.Behavioral and pathologic changes of chronic cerebral hypoperfusion in mice[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(09):813-817.
段炜,郑健,桂莉,田洪.小鼠慢性脑血流低灌注状态的行为学检测及病理学观察[J].陆军军医大学学报(原第三军医大学学报),2009,31(09):813-817. DOI：

　DUAN Wei,ZHENG Jian,GUI Li,TIAN Hong.Behavioral and pathologic changes of chronic cerebral hypoperfusion in mice[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(09):813-817. DOI：

摘要

目的观察小鼠慢性脑血流低灌注状态下行为学及其病理学变化。方法 62只C57BL/6小鼠均分为模型组和假手术组。模型组：采用内径为0.18 mm的微型弹簧圈套C57BL/6小鼠双侧颈总动脉，造成血管狭窄导致全脑血流低灌注状态；假手术组：仅暴露双侧颈总动脉，不圈套微型弹簧。在成模30 d时两组各取8只小鼠，采用八臂迷宫实验检测小鼠认知功能的改变，并在成模7、14 d和30 d时分别处死动物，采用Kluver-Barrera染色观察神经纤维改变及免疫组化观察胶质细胞增生程度，同时用Evans Blue荧光观察血脑屏障通透性的变化。结果建模30 d后模型组八臂迷宫实验工作记忆错误显著多于假手术组（P0.05）。在成模7 d时未见显著病理变化，在14、30 d时观察到模型组脑白质区域神经纤维稀疏[胼胝体神经纤维损伤分级分别为（1.13±0.05）和（1.96±0.08）

P0.05]，星形胶质细胞和小胶质细胞显著增生[胼胝体星型胶质细胞计数分别为（104.52±12.31）和（192.57±24.23），小胶质细胞计数分别为（98.25±15.27）和（172.36±18.29），P0.01]，Evans Blue荧光观察到脑内微血管血液成分外渗。结论慢性脑血流低灌注状态导致小鼠的认知功能下降、胼胝体等白质神经纤维稀疏及胶质细胞增生。

Abstract

Objective To observe behavioral characteristics and pathologic changes of chronic cerebral hypoperfusion in mice. Methods Totally 62 adult C57BL/6 mice were subjected to either sham-operation (n=31) or bilateral common carotid artery stenosis using external microcoils with an inner diameter of 0.18 mm(n=31). At 30 d after the stenosis

the animals of the 2 groups (8 mice for each group) underwent behavioral test of 8-arm radial maze. In 7

14 and 30 d

the rest mice were sacrificed for their brain tissue samples which were examined with Kluver-Barrera staining and immunohistochemical assay for markers of microglia and astroglia

glial fibrillary acidic protein (GFAP) and CD11b. In 14 d after the model establishment

Evans blue dye extravasation test was performed for the blood-brain barrier function. Results The model group made significantly more errors than sham-operated group in 8-arm radial maze test at 30 d after the surgery. White matter lesions occurred and the proliferation of activated microglia and astroglia were observed in white matter in model mice after 14 and 30 d bilateral common carotid artery stenosis. The disruption of blood-brain barrier function of model mice was indicated in the evans blue extravasation test at 14 d after bilateral common carotid artery stenosis. Conclusion Cognitive impairment

white matter lesions and glial activation are successfully induced after bilateral common carotid artery stenosis in mice model of chronic cerebral hypoperfusion.

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