Study on piezoelectric quartz crystal microarray immunosensor for the detection of Agkistrodon acutus venom

刘明华; 姚婕; 向强; 张波; 府伟灵

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Study on piezoelectric quartz crystal microarray immunosensor for the detection of Agkistrodon acutus venom

更新时间：2026-01-09

- Study on piezoelectric quartz crystal microarray immunosensor for the detection of Agkistrodon acutus venom
- Journal of Army Medical University Vol. 31, Issue 12, Pages: 1158-1160(2009)
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  CLC： R312;R446.69;R996.3
- Published：2009
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刘明华,姚婕,向强,张波,府伟灵.压电免疫传感器阵列检测尖吻蝮蛇毒的初步研究[J].陆军军医大学学报(原第三军医大学学报),2009,31(12):1158-1160.

　LIU Ming-hua,YAO Jie,XIANG Qiang,ZHANG Bo,FU Wei-ling.Study on piezoelectric quartz crystal microarray immunosensor for the detection of Agkistrodon acutus venom[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(12):1158-1160.
刘明华,姚婕,向强,张波,府伟灵.压电免疫传感器阵列检测尖吻蝮蛇毒的初步研究[J].陆军军医大学学报(原第三军医大学学报),2009,31(12):1158-1160. DOI：

　LIU Ming-hua,YAO Jie,XIANG Qiang,ZHANG Bo,FU Wei-ling.Study on piezoelectric quartz crystal microarray immunosensor for the detection of Agkistrodon acutus venom[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(12):1158-1160. DOI：

摘要

目的探讨以压电传感阵列技术检测尖吻蝮蛇毒的可行性。方法构建2×5 的AT切向、基频10 MHz 的石英晶体微阵列，将尖吻蝮蛇毒抗体巯基化

通过自组装技术固定在石英晶体金膜电极表面，检测不同浓度的尖吻蝮蛇毒标准液。结果尖吻蝮蛇毒压电免疫传感器阵列上，最适抗体固定浓度为3 μg/ml，尖吻蝮蛇毒与相应抗体反应时间为40 min，检测尖吻蝮蛇毒最低浓度为0.1 μg/ml

蛇毒浓度在0.1～4.0 μg/ml范围时

与晶体频率变化之间呈良好的线性关系。结论采用压电免疫传感器阵列检测尖吻蝮蛇毒响应特性良好，具有灵敏度高、操作简单、不需标记等优点

是实现快速仪器化检测蛇伤的可能途径。

Abstract

Objective To explore the possibility of piezoelectric microarray immunosensor for the detection of Agkistrodon acutus venom. Methods Microarray immunosensor with quartz crystal of 10 MHz AT-cut and 2×5 gold-coated electrodes was prepared. The thiol-treated venom antibody was immobilized by a self assembling device for the detection of the standard fluid for different concentrations of the venom. Results Experimental results showed that the optimal concentration of the antibody was 3.0 g/ml with the response time of 40 minutes. The piezoelectric immunosensor could well respond to homologous venoms. Within the range of 0.1~4.0 g/ml

the frequency shifts were linearly dependent on the venom concentration. Conclusion Piezoelectric microarray immunosensor for the detection of Agkistrodon acutus venom is of high specificity of response

high sensitivity

and simple operation without marking. The technique of piezoelectric microarray immunosensor is possible to test snakebite quickly

quantitatively

and instrumentally.

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