Objective    To evaluate the reliability and accuracy of rat model of acute cerebral ischemia-reperfusion in living condition by using CT perfusion imaging (CTPI) technology.     Methods    A stable and reversible acute cerebral ischemia-reperfusion model with unilateral middle cerebral occlusion was established in Wistar rats and evaluated by CTPI

TTC staining and HE staining. There were 2 groups: ischemia group and ischemia+reperfusion group.      Results    ①Cerebral blood flow (CBF) core areas and cerebral blood volume (CBV) of low volume infusion areas on CTPI showed no significant difference at various time points and between the 2 groups (P0.05)

and the results obtained from CTPI were concordant  with those from TTC staining for infarction zone (P0.05)

but significantly different with the ischemic volume of average transit time (MTT) (P0.05). There were significant differences between MTT ischemic volume and infarction zone volume by TTC staining (P0.05). ②There was correlation between CTPI parameters and infarction volume by TTC staining

and CBV relevance was the best (r=0.74). ③CBF and CBV in infarction zone were significantly lower than those in contralateral control zone

and MTT extended; CBF in ischemic penumbra (IP) zone was significantly lower than that in contralateral control zone

while CBV was normal or higher

and MTT extended slightly; CBF

CBV and MTT in relatively normal zone had no obvious changes than these on contralateral sides. ④The brain tissue with the matched decrease in CBF and CBV showed the performance-based nerve cell death in HE staining

and the brain tissue with CBF decreased and CBV normal showed the performance-based nerve cell degeneration.     Conclusion    CTPI as a rapid

accurate

non-invasive method can evaluate the brain homodynamics of cerebral ischemia-reperfusion animal model in living state

and can display the site

size

extent of ischemic region during cerebral infarction

and has important clinical value for the ultra-acute cerebral infarction in treatment and review.