Effects of DEX and rhBMP2 on alkaline phosphatase activity of human dental pulp cells in vitro

张慧宇; 向学熔; 范小平

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Effects of DEX and rhBMP2 on alkaline phosphatase activity of human dental pulp cells in vitro

更新时间：2026-01-09

- Effects of DEX and rhBMP2 on alkaline phosphatase activity of human dental pulp cells in vitro
- Journal of Army Medical University Vol. 31, Issue 13, Pages: 1264-1267(2009)
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  CLC： R345;R446.19;R78
- Published：2009
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张慧宇,向学熔,范小平.DEX和rhBMP2对体外培养人牙髓细胞碱性磷酸酶活性的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(13):1264-1267.

　Hui-yu,XIANG Xue-rong,FAN Xiao-ping.Effects of DEX and rhBMP2 on alkaline phosphatase activity of human dental pulp cells in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(13):1264-1267.
张慧宇,向学熔,范小平.DEX和rhBMP2对体外培养人牙髓细胞碱性磷酸酶活性的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(13):1264-1267. DOI：

　Hui-yu,XIANG Xue-rong,FAN Xiao-ping.Effects of DEX and rhBMP2 on alkaline phosphatase activity of human dental pulp cells in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(13):1264-1267. DOI：

摘要

目的探讨不同浓度地塞米松(Dexamethasone，DEX)、重组人骨形态发生蛋白2(recombinant human bone morphogenetic protein 2

rhBMP2)及两者联合应用对体外培养的人牙髓细胞(human dental pulp cells，HDPCs)碱性磷酸酶(Alkaline Phosphatase，ALP)活性的影响。方法组织块法培养HDPCs并进行鉴定；采用酶动力学的方法，观察DEX、rhBMP2 及二者联合应用对HDPCs 的ALP活性的影响。结果单独应用DEX时ALP活性明显增高，且在生理浓度范围内当浓度为0.01 nmol/ml时，对HDPCs的ALP活性达到最大的刺激作用，rhBMP2对HDPCs的ALP活性呈浓度依赖性增强；当联合应用DEX和rhBMP2，ALP活性比单独应用相应浓度的rhBMP2明显增高。结论 DEX、rhBMP2对HDPCs的ALP活性均有增强作用，同时二者对HDPCs的ALP活性有显著的功能放大性协同增强作用。

Abstract

Objective To study the effects of dexamethasone (DEX)

recombinant bone morphogenetic protein-2 (rhBMP2)

and combined application of rhBMP2 and DEX on alkaline phosphatase (ALP) activity of human dental pulp cells (HDPCs) in vitro. Methods HDPCs were cultured by tissue block method and identified. Effects of DEX

rhBMP2

and combined application of both on ALP activity of HDPCs were determined by a modified enzyme dynamical method. Results DEX could enhance ALP activity

reaching the peak value at the concentration of 0.01 nmol/ml. rhBMP2 could enhance ALP activity in a dose-dependent manner. ALP activity was significantly higher under the condition of combined application of DEX and rhBMP2 than single application of DEX or rhBMP2 only. Conclusion Both DEX and rhBMP2 can enhance ALP activity of HDPCs. However

combined application of DEX and rhBMP2 can greatly enhance ALP activity of HDPCs.

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