siRNA targeting STAT5 induces apoptosis in human hepatocellular carcinoma cell line SMMC-7721

赵振军; 张丽杰; 鹿刚; 张引娟; 单保恩

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siRNA targeting STAT5 induces apoptosis in human hepatocellular carcinoma cell line SMMC-7721

更新时间：2026-01-09

- siRNA targeting STAT5 induces apoptosis in human hepatocellular carcinoma cell line SMMC-7721
- Journal of Army Medical University Vol. 31, Issue 12, Pages: 1147-1150(2009)
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  CLC： R73-362;R735.7
- Published：2009
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赵振军,张丽杰,鹿刚,张引娟,单保恩.靶向STAT5的siRNA诱导人肝癌SMMC-7721细胞凋亡[J].陆军军医大学学报(原第三军医大学学报),2009,31(12):1147-1150.

　ZHAO Zhen-jun,ZHANG Li-jie,LU Gang,ZHANG Yin-juan,SHAN Bao-en.siRNA targeting STAT5 induces apoptosis in human hepatocellular carcinoma cell line SMMC-7721[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(12):1147-1150.
赵振军,张丽杰,鹿刚,张引娟,单保恩.靶向STAT5的siRNA诱导人肝癌SMMC-7721细胞凋亡[J].陆军军医大学学报(原第三军医大学学报),2009,31(12):1147-1150. DOI：

　ZHAO Zhen-jun,ZHANG Li-jie,LU Gang,ZHANG Yin-juan,SHAN Bao-en.siRNA targeting STAT5 induces apoptosis in human hepatocellular carcinoma cell line SMMC-7721[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(12):1147-1150. DOI：

摘要

目的采用RNA干扰技术阻断STAT5基因的表达，观察其对人肝癌SMMC-7721凋亡的影响。方法构建3种靶向STAT5 的小干扰RNA (siRNA)真核表达载体，采用Lipofectamine

2000 转染肝癌细胞SMMC-7721；分别采用半定量RT-PCR、Western blot技术检测转染前后SMMC-7721 细胞STAT5 mRNA和蛋白质表达的变化；采用透射电镜技术观察细胞形态变化；流式细胞术检测细胞凋亡率。结果 3条靶向STAT5 的序列特异性的siRNA可以有效地抑制SMMC-7721细胞STAT5 mRNA和蛋白质表达，STAT5 mRNA表达抑制率分别为70.43%、43.02%、45.07%，STAT5蛋白表达抑制率分别为67.45%、37.36%、41.86%；转染靶向STAT5的siRNA 真核表达载体48 h后出现凋亡细胞的典型形态学变化，并诱导25.61%的SMMC-7721细胞发生凋亡。结论靶向STAT5的siRNA 真核表达载体可以有效、特异地阻断SMMC-7721 细胞STAT5基因的表达，并能够诱导SMMC-7721细胞凋亡，STAT5 siRNA在人肝癌的基因治疗中可能具有潜在的应用价值。

Abstract

Objective To investigate the effects of inhibition of STAT5 gene expression by RNA interference technology on apoptosis of human hepatocellular carcinoma cell line SMMC-7721. Methods Three siRNA eukaryotic expression vectors against STAT5 were constructed and transfected with lipofectamine

2000 into SMMC-7721 cells. The changes in STAT5 expression were detected by semi-quantitative RT-PCR and Western blot. Cell apoptosis was assayed by flow cytometry (FCM). Results The sequence-specific siR

NA could effectively and specifically inhibit STAT5 gene expression at both mRNA and protein levels. The inhibition rates of STAT5 mRNA expression were 70.43%

43.02%

and 45.07%

respectively. The inhibition rates of STAT5 protein expression were 67.45%

37.36%

and 41.86%

respectively. At 48 h after transfection

apoptosis rate was 25.61%. Conclusion siRNA against STAT5 can inhibit STAT5 gene expression in SMMC-7721 cells effectively and specifically and induce apoptosis of SMMC-7721 cells. siRNA targeting STAT5 has a great potential value in gene therapy of hepatocellular carcinoma.

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