Effect of CXCR4/SDF-1 on proliferation and migration of human nasopharyngeal carcinoma cell lines

邱际华; 刘求真; 陈丽; 焦锋; 姚开泰

您当前的位置：

首页 >

文章列表页 >

Effect of CXCR4/SDF-1 on proliferation and migration of human nasopharyngeal carcinoma cell lines

更新时间：2026-01-09

- Effect of CXCR4/SDF-1 on proliferation and migration of human nasopharyngeal carcinoma cell lines
- Journal of Army Medical University Vol. 31, Issue 7, Pages: 598-600(2009)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R329.28;R73-37;R739.63
- Published：2009
- 稿件说明：
移动端阅览
邱际华,刘求真,陈丽,焦锋,姚开泰.CXCR4/SDF-1对鼻咽癌细胞增殖与迁移能力的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(07):598-600.

　QIU Ji-hua,LIU Qiu-zhen,CHEN Li,JIAO Feng,YAO Kai-tai.Effect of CXCR4/SDF-1 on proliferation and migration of human nasopharyngeal carcinoma cell lines[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(07):598-600.
邱际华,刘求真,陈丽,焦锋,姚开泰.CXCR4/SDF-1对鼻咽癌细胞增殖与迁移能力的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(07):598-600. DOI：

　QIU Ji-hua,LIU Qiu-zhen,CHEN Li,JIAO Feng,YAO Kai-tai.Effect of CXCR4/SDF-1 on proliferation and migration of human nasopharyngeal carcinoma cell lines[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(07):598-600. DOI：

摘要

目的观察鼻咽癌不同恶性程度的细胞中趋化因子受体CXCR4 的表达

检测CXCR4/SDF- 1 反应轴对鼻咽癌细胞的增殖作用

SDF- 1对CXCR4阳性肿瘤细胞的趋化作用

探讨 CXCR4 /SDF-1生物学轴对人鼻咽癌细胞增殖与迁移能力的影响。方法以鼻咽癌成瘤高转移细胞株5-8F及成瘤不转移细胞株6-10B为研究对象，采用Western blot免疫印迹法检测鼻咽癌细胞株CXCR4蛋白的表达情况，SDF-1及CXCR4阻断剂AMD3100作用于两种细胞后

MTT法检测细胞的增殖能力

体外迁徙实验检测 CXCR4/SDF- 1反应轴对鼻咽癌细胞的趋化作用。结果 5-8F细胞株CXCR4蛋白的表达明显高于6-10B细胞株； SDF-1能明显增强5-8F细胞增殖与迁移能力， CXCR4阻断剂AMD3100作用后

5-8F细胞增殖与迁移能力明显降低；SDF-1对6-10B细胞的迁移及增殖能力无明显影响。结论 CXCR4 /SDF-1生物学轴与鼻咽癌细胞株的增殖与迁移有一定的关系，AMD3100可明显抑制鼻咽癌细胞的增殖与迁移能力。

Abstract

Objective To detect the expression of chemokine receptors 4 (CXCR4) in human nasopharyngeal carcinoma cell lines (NPC cells) and explore the role of CXCR4/stromal cell-derived factor-1 (SDF-1) axis in the proliferation and migration of tumor cell. Methods The expression of CXCR4 protein in NPC cell lines 5-8F (with high tumogenicity and metastasis) and 6-10B (having tumogenicity but no metastasis) was measured by Western blot analysis. After the addition of SDF-1 and CXCR4 blocker

AMD3100

the changes of cell growth were observed by MTT. The changes of cell migration were measured by Transwell. Results Western blotting showed that 5-8F cells expressed higher CXCR4 levels than 6-10B cells. The growth rate and the number of migration cells in 5-8F cells was increased after SDF-1 treatment

and significantly decreased after the addition of AMD3100. However

6-10B cells had no response to SDF-1. Conclusion CXCR4/SDF-1 axis may play an important role in NPC cells proliferation and migration

and CXCR4 blocker can efficiently suppress this effect.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Expression of β-arrestin1 in oral squamous cell carcinoma and its effect on cell proliferation, migration and invasion

Effect of RNA sensor IFIH1 on proliferation, migration and invasion of HPV positive cervical cancer cells

Anti-gastric cancer effect of a novel small molecule inhibitor 1-3-51 targeting FOXM1 in vitro

LINC00525 targeting miR-338-3p promotes apoptosis and reduces paclitaxel resistance in gastric cancer cells

Sphingosine-1-phosphate type 1 receptor aggravates neointimal hyperplasia after carotid artery injury in mice

Related Author

HAO Xiaohui

CHEN Min

WU Nan

DING Yunshan

ZHU Lifan

DAI Haitao

喻琳

罗莹

Related Institution

School of Medicine， Shihezi University

Department of Stomatology， the First Affiliated Hospital of Shihezi University

Department of Gastroenterology, Qingdao Central Hospital of Rehabilitation University

Endoscopy Center, Qingdao Central Hospital of Rehabilitation University

Department of Pathology， Second Affiliated Hospital， Army Medical University （Third Military Medical University）

⁰