PCR analysis of clinically isolated Staphylococcus aureus biofilm associated gene

孙凤军; 枉前; 夏培元

您当前的位置：

首页 >

文章列表页 >

PCR analysis of clinically isolated Staphylococcus aureus biofilm associated gene

更新时间：2026-01-09

- PCR analysis of clinically isolated Staphylococcus aureus biofilm associated gene
- Journal of Army Medical University Vol. 31, Issue 15, Pages: 1447-1449(2009)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R378.11;R446.5
- Published：2009
- 稿件说明：
移动端阅览
孙凤军,枉前,夏培元.临床分离金黄色葡萄球菌生物膜相关基因的PCR分析[J].陆军军医大学学报(原第三军医大学学报),2009,31(15):1447-1449.

　SUN Feng-jun,WANG Qian,XIA Pei-yuan.PCR analysis of clinically isolated Staphylococcus aureus biofilm associated gene[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(15):1447-1449.
孙凤军,枉前,夏培元.临床分离金黄色葡萄球菌生物膜相关基因的PCR分析[J].陆军军医大学学报(原第三军医大学学报),2009,31(15):1447-1449. DOI：

　SUN Feng-jun,WANG Qian,XIA Pei-yuan.PCR analysis of clinically isolated Staphylococcus aureus biofilm associated gene[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(15):1447-1449. DOI：

摘要

目的确定临床分离的金黄色葡萄球菌生物膜形成能力和生物膜相关基因之间的关系，为生物膜形成的机制研究提供依据。方法 96孔板番红染色法检测生物膜的形成，icaAD、icaBC、sar、agr和sigB的PCR扩增，并对产物进行测序和同源性比较。结果 27株金黄色葡萄球菌中共有17株形成了肉眼可见的生物膜，其中以X387和X409两株最明显；有22株扩增出icaAD和icaBC，全部菌株扩增出sar、agr和sigB。结论 ica是形成金葡菌生物膜的关键基因，但ica在形成生物膜过程中需要其他基因的协同作用。

Abstract

Objective To investigate the correlation of biofilm forming ability with biofilm associated gene of the clinically isolated Staphylococcus aureus for providing basis for the further studies of the mechanisms of biofilm formation. Methods Safranine staining was conducted for the detection of the forming ability of biofilm in 96-well plates. icaAD

icaBC

sar

agr and sigB was amplified by PCR. Results Formation of biofilm could be found macroscopically in 17 out of 27 strains

especially to X387 and X409. icaAD and icaBC were amplified in 22 isolates and sar

agr and sigB in all 27 S. aureus strains. Conclusion ica operon is the key gene for biofilm formation but cooperative action of other genes is needed during the process of biofilm formation.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

WalK(S221P) mutation promotes production of Staphylococcus aureus capsule

Construction of Staphylococcus aureus bioluminescence tracing system based on Antares2

Inhibitory effect of emodin on MRSA biofilm and its mechanism

Antibacterial activity and mechanism of antimicrobial peptide ET12 against Gram-positive bacteria

Therapeutic effect of polyclonal lipoprotein SA2275 antibodies on Staphylococcus aureus-induced brain abscess in mice

Related Author

郭祖文

饶一凡

彭华刚

饶贤才

胡珍

尚伟龙

饶贤才

刘婧怡

Related Institution

No data

⁰