Effects of Panax notoginseng saponins on mRNA expressions of ABCA1 and LXRα in foam cells

李卓莹; 何雪峰; 张海港; 贾乙; 李晓辉

您当前的位置：

首页 >

文章列表页 >

Effects of Panax notoginseng saponins on mRNA expressions of ABCA1 and LXRα in foam cells

更新时间：2026-01-09

- Effects of Panax notoginseng saponins on mRNA expressions of ABCA1 and LXRα in foam cells
- Journal of Army Medical University Vol. 31, Issue 7, Pages: 592-594(2009)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC： R285.5;R329.25;R392.12
- Published：2009
- 稿件说明：
移动端阅览
李卓莹,何雪峰,张海港,贾乙,李晓辉.三七总皂苷对泡沫细胞内ABCA1和LXRα mRNA表达的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(07):592-594.

　LI Zhuo-ying,HE Xue-feng,ZHANG Hai-gang,JIA Yi,LI Xiao-hui.Effects of Panax notoginseng saponins on mRNA expressions of ABCA1 and LXRα in foam cells[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(07):592-594.
李卓莹,何雪峰,张海港,贾乙,李晓辉.三七总皂苷对泡沫细胞内ABCA1和LXRα mRNA表达的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(07):592-594. DOI：

　LI Zhuo-ying,HE Xue-feng,ZHANG Hai-gang,JIA Yi,LI Xiao-hui.Effects of Panax notoginseng saponins on mRNA expressions of ABCA1 and LXRα in foam cells[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(07):592-594. DOI：

摘要

目的观察三七总皂苷(Panax notoginseng saponins

PNS)对泡沫细胞ABCA1和LXRα mRNA表达及脂质沉积的影响。方法提取SD大鼠腹腔巨噬细胞纯化后，与高、中、低剂量PNS（80、40、20 μg/ml）及氧化低密度脂蛋白共孵育48 h。油红O染色观察细胞内脂质堆积情况，酶法测定细胞内总胆固醇(TC)、游离胆固醇(FC)、胆固醇酯(CE)的含量，并计算CE/TC值。采用RT-PCR法检测泡沫细胞ABCA1和LXRα mRNA表达情况。结果模型组细胞中TC、FC、CE含量及CE/TC值均显著高于对照组（P0.05）；与模型组相比，PNS高、中剂量组细胞内TC、FC、CE含量及CE/TC值均显著降低(P0.05)。PNS高、中剂量组中ABCA1及LXRα mRNA的表达均显著高于模型组(P0.01)。结论 PNS可以上调ABCA1和LXRα mRNA表达并减少巨噬细胞源性泡沫细胞中脂质沉积。

Abstract

Objective To observe the effects of Panax notoginseng saponins (PNS) on mRNA expressions of ABCA1 and LXRα and lipid deposition in foam cells. Methods Peritoneal macrophages obtained from SD rats after purification were incubated with ox-LDL for 48 h to establish foam cell model. PNS (20

80 μg/ml

respectively) were given for intervention. The cellular lipid accumulation was examined by oil red staining. The cellular contents of total cholesterol (TC)

free cholesterol (FC) and cholesterol ester (CE) and the ratio of CE/TC were detected by enzymic methods. The mRNA levels of ABCA1 and LXRα were determined by RT-PCR. Results The contents of TC

CE and CE/TC ratio in model group were significantly increased compared with control group (P0.05). Compared with model group

40 and 80 μg/ml PNS reduced the contents of TC

CE and CE/TC ratio remarkably (P0.05) and increased ABCA1 and LXRα mRNA expressions significantly (P0.01). Conclusion PNS reduces lipid deposition and upregulates ABCA1 and LXRα mRNA expressions in foam cells.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Panax notoginseng saponins suppresses mTOR signaling pathway in K562 cells

Chronic renal failure influences macrophages’ cholesterol content by down-regulating ABCA1

Effect of rosiglitazone on expression of acyl-coenzyme A cholesterol acyltransferase 1 and ATP-binding cassette transporter A1 in foam cells

Mongolian drug “Tonglaga-5” improves glucose uptaking in 3T3-L1 adipocytes

Differentiation of bone marrow mesenchymal stem cells into cardiomyocytes with FGF-2 and Panax notoginseng saponins

Related Author

李见

王露

于北凯

杨向荣

李玉云

翟玮玮

汤为学

陈力学

Related Institution

No data

⁰