Study on biological signal amplification system of leaky surface acoustic wave sensor

王云霞; 张立群; 罗阳; 丁毅; 施建峰; 徐清华; 汪广杰; 曹亮; 陈鸣; 府伟灵

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Study on biological signal amplification system of leaky surface acoustic wave sensor

更新时间：2026-01-09

- Study on biological signal amplification system of leaky surface acoustic wave sensor
- Journal of Army Medical University Vol. 31, Issue 11, Pages: 1013-1016(2009)
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  CLC： R318.04;R394-33;R446
- Published：2009
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王云霞,张立群,罗阳,丁毅,施建峰,徐清华,汪广杰,曹亮,陈鸣,府伟灵.漏声表面波传感器生物信号放大系统的研究[J].陆军军医大学学报(原第三军医大学学报),2009,31(11):1013-1016.

　WANG Yun-xia,ZHANG Li-qun,LUO Yang,DING Yi,SHI Jian-feng,XU Qing-hua,WANG Guang-jie,CAO Liang,CHEN Ming,FU Wei-ling.Study on biological signal amplification system of leaky surface acoustic wave sensor[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(11):1013-1016.
王云霞,张立群,罗阳,丁毅,施建峰,徐清华,汪广杰,曹亮,陈鸣,府伟灵.漏声表面波传感器生物信号放大系统的研究[J].陆军军医大学学报(原第三军医大学学报),2009,31(11):1013-1016. DOI：

　WANG Yun-xia,ZHANG Li-qun,LUO Yang,DING Yi,SHI Jian-feng,XU Qing-hua,WANG Guang-jie,CAO Liang,CHEN Ming,FU Wei-ling.Study on biological signal amplification system of leaky surface acoustic wave sensor[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(11):1013-1016. DOI：

摘要

目的研究利用“RecA蛋白-互补单链DNA”探针与bis-PNA/dsDNA复合物相结合，作为生物信号放大系统提高传感器检测灵敏度的可行性。方法漏声表面波传感器检测通道金膜表面先固定针对人乳头瘤病毒(HPV)的bis-PNA探针，加入HPV基因组DNA与之完全反应后，再加入不同浓度的“RecA蛋白-互补单链DNA”探针，在反应过程中加入ATPγS提供能量，分别探索优化RecA蛋白及ATPγS的最佳反应浓度。结果当RecA蛋白浓度为45 μg/ml，所引起的相位变化值为(11.74±1.03)°，显著高于其他浓度组（P0.01）；在最佳RecA蛋白浓度下，当ATPγS浓度为2.5 mmol/L时，所引起的相位变化值为(10.71±0.73)°，显著高于其他ATPγS浓度组（P0.01）。结论 “RecA蛋白-互补单链DNA”探针复合体与bis-PNA/dsDNA复合物相结合可以有效提高传感器的检测灵敏度，并明显缩短检测时间。

Abstract

Objective To study the feasibility of combination of “RecA protein-complementary single strand DNA” probe and bis-PNA/dsDNA complexes as the biological signal amplification system for the improvement of the sensitivity of leaky surface acoustic wave bis-peptide nucleic acid biosensor. Methods Bis-PNA probe for detecting human papilloma virus (HPV) was immobilized on the surface of gold membrane of the detection channel. After the probe was hybridized with the corresponding target DNA completely

different concentrations of “RecA protein-complementary single strand DNA probe” were added to react with bis-PNA/dsDNA complexes. Results The phase shift value was (11.74±1.03) deg at the concentration of 45 μg/ml of RecA protein

significantly different as compared with other concentrations (P0.01). At the optimal concentration of RecA protein

the phase shift value was (10.71±0.73) deg at the concentration of 2.5 mmol/L of ATPγS

significantly different as compared with other concentrations (P0.01). Conclusion Combination of “RecA protein-complementary single strand DNA probe” and bis-PNA/dsDNA complexes contributes to effective improvement of the sensitivity and significantly shortened detection time.

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