Condition optimization for modification of recombinant human ciliary neurotrophic factor with polythylene glycol

王彦; 范开; 夏永鹏; 张继兰; 李秀梅; 邱宗荫

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Condition optimization for modification of recombinant human ciliary neurotrophic factor with polythylene glycol

更新时间：2026-01-09

- Condition optimization for modification of recombinant human ciliary neurotrophic factor with polythylene glycol
- Journal of Army Medical University Vol. 31, Issue 14, Pages: 1345-1348(2009)
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  CLC： R914;R966;R977.6
- Published：2009
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王彦,范开,夏永鹏,张继兰,李秀梅,邱宗荫.重组人睫状神经因子聚乙二醇化条件的优化[J].陆军军医大学学报(原第三军医大学学报),2009,31(14):1345-1348.

　WANG Yan,FAN Kai,XIA Yong-peng,ZHANG Ji-lan,LI Xiu-mei,QIU Zong-yin.Condition optimization for modification of recombinant human ciliary neurotrophic factor with polythylene glycol[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(14):1345-1348.
王彦,范开,夏永鹏,张继兰,李秀梅,邱宗荫.重组人睫状神经因子聚乙二醇化条件的优化[J].陆军军医大学学报(原第三军医大学学报),2009,31(14):1345-1348. DOI：

　WANG Yan,FAN Kai,XIA Yong-peng,ZHANG Ji-lan,LI Xiu-mei,QIU Zong-yin.Condition optimization for modification of recombinant human ciliary neurotrophic factor with polythylene glycol[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(14):1345-1348. DOI：

摘要

目的对单甲氧基聚乙二醇丁醛（mPEG-ButyrALD，相对分子质量20×10

）修饰重组人睫状神经因子（rh-CNTF）的条件进行优化。方法优化了pH值、温度、时间、rh-CNTF与PEG的修饰比例、rh-CNTF浓度等修饰条件，并对修饰产物行分离纯化。鸡胚背根节培养法检测和比较rh-CNTF 和PEG-rhCNTF的体外生物学活性。结果 rh-CNTF与PEG的修饰比例、rh-CNTF的浓度和pH值是主要影响因素，最佳pH为6.5，最佳rh-CNTF浓度为1～2 mg/ml，rh-CNTF与 PEG的最佳质量比为1∶1，时间和温度影响甚微。rh-CNTF的体外神经营养活性约为PEG-rhCNTF的2倍。结论本法优化了修饰重组人睫状神经因子的条件。

Abstract

Objective To optimize the modification condition of recombinant human ciliary neurotrophic factor (rh-CNTF) with mPEG-ButyrALD (relative molecular weight 20×10). Methods Different pH values

temperatures

times and the mass ratio of rh-CNTF and PEG

the concentration of rh-CNTF was used to obtain better rh-CNTF PEGylation. And then the modified rh-CNTF was separated and purified. The in vitro biological activity of rh-CNTF and PEG rhCNTF was measured by chicken embryo dorsal root ganglion (DRG) culture. Results The mass ratio of rh-CNTF to PEG

rh-CNTF concentration and pH value showed significant effect on rh-CNTF PEGylation

while time and temperature had little influence on the modification effect. The optimal pH value was 6.5

concentration of rh-CNTF was 1 to 2 mg/ml

and mass ratio of rh-CNTF and PEG was 1∶1 on modification condition. The in vitro biological activity of rh-CNTF was about 2 times higher than that of PEG-rhCNTF in chicken embryo dorsal root ganglion (DRG) culture. Conclusion Optimized parameters is obtained to modify rh-CNTF with mPEG-ButyrALD.

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