Effects of BMP-2 on dopaminergic neuronal differentiation of neural stem cells from SVZa and on DLX5 expression

刘胜华; 周政; 杨辉; 刘俊; 阴金波; 辛志成

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Effects of BMP-2 on dopaminergic neuronal differentiation of neural stem cells from SVZa and on DLX5 expression

更新时间：2026-01-09

- Effects of BMP-2 on dopaminergic neuronal differentiation of neural stem cells from SVZa and on DLX5 expression
- Journal of Army Medical University Vol. 31, Issue 6, Pages: 480-483(2009)
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  CLC： R322.8;R329.2;R394.2
- Published：2009
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刘胜华,周政,杨辉,刘俊,阴金波,辛志成.BMP-2对室管膜前下区神经干细胞向多巴胺能神经元分化及对DLX5表达的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(06):480-483.

　LIU Sheng-hua,ZHOU Zheng,YANG Hui,LIU Jun,YIN Jin-bo,XIN Zhi-cheng.Effects of BMP-2 on dopaminergic neuronal differentiation of neural stem cells from SVZa and on DLX5 expression[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(06):480-483.
刘胜华,周政,杨辉,刘俊,阴金波,辛志成.BMP-2对室管膜前下区神经干细胞向多巴胺能神经元分化及对DLX5表达的影响[J].陆军军医大学学报(原第三军医大学学报),2009,31(06):480-483. DOI：

　LIU Sheng-hua,ZHOU Zheng,YANG Hui,LIU Jun,YIN Jin-bo,XIN Zhi-cheng.Effects of BMP-2 on dopaminergic neuronal differentiation of neural stem cells from SVZa and on DLX5 expression[J].J Amry Med Univ (J Third Mil Med Univ),2009,31(06):480-483. DOI：

摘要

目的探讨骨形成蛋白2（bone morphogenetic protein-2，BMP-2）对室管膜前下区(anterior subventricular zone，SVZa)神经干细胞( neural stem cells，NSCs)向多巴胺能神经元分化及对DLX5表达的影响。方法体外培养SVZa NSCs，分为对照组、BMP-2组、BMP-2＋Noggin组和Noggin组，通过免疫荧光染色和流式细胞术，观察其分化为酪氨酸羟化酶阳性（TH

）神经元的情况；用RT-PCR检测DLX5 mRNA表达水平。结果 BMP-2组SVZa NSCs向TH

神经元分化的比例和DLX5 mRNA表达水平均显著高于对照组（P0.05），且这种效应能被其拮抗剂Noggin特异性抑制。结论 BMP-2可能通过上调DLX5表达水平从而促进SVZa NSCs向多巴胺能神经元分化。

Abstract

Objective To investigate the effect of bone morphogenetic protein-2 (BMP-2) on dopaminergic neuronal differentiation of neural stem cells (NSCs) of anterior subventricular zone (SVZa) and expression of DLX5. Methods The neural stem cells from SVZa were separated and cultured in vitro

then divided into control group

BMP-2 group

BMP-2+Noggin group

Noggin group. TH

neuronal differentiation of neural stem cells from SVZa was identified by immunofluorescence staining and flow cytometry. RT-PCR was employed to assay the expression of DLX5 mRNA. Results Both the percentage of TH

neurons and expression of DLX5 mRNA in BMP-2 group were much higher than those in control group (P0.05). And those effects could be specifically blocked by BMP-2 antagonist Noggin. Conclusion BMP-2 may promote dopaminergic neuronal differentiation of neural stem cells of SVZa through upregulating DLX5.

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